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DOI :
10.3791/50195-v
August 8th, 2013
Chapters
0:05
Title
1:44
Metabolic Labeling with 4-thiouridine
3:05
Thiol-specific Biotinylation of Newly Transcribed RNA
7:00
Recovery of Newly Transcribed RNA
8:17
Results: Metabolic Labeling of Newly Transcribed RNA
10:25
Conclusion
5:09
Separation of Labeled and Unlabeled RNA Using Streptavidin-coated Magnetic Beads
全細胞RNAは、短期的RNA合成と崩壊の変化と同様に、RNAプロセシングの動態を研究するための貧乏人のテンプレートが用意されています。ここでは、これらの制限を克服することができ、新たに転写されたRNAのチオール特有のビオチン化および精製し、4 - チオウリジンと新しく転写されたRNAの代謝標識を記述します。
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