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DOI :
10.3791/50200-v
March 13th, 2013
Chapters
0:05
Title
1:38
Preparing Primary Melanoma Single-cells from Tumor Tissue
4:33
Characterization of Primary Cell Culture and Fibroblast Depletion
5:44
Magnetic Cell Sorting of CD133+ and CD133- Melanoma Cells
9:27
Results: Magnetic Cell Sorting of CD133+ and CD133- Melanoma Cells
11:38
Conclusion
この記事では、初代培養細胞に新たに取得したメラノーマ組織の調製を記述する方法、および腫瘍細胞から赤血球および線維芽細胞の汚染物を除去する。最後に、我々はどのように説明したCD133 +推定メラノーマ幹細胞は、CD133から順にソートされ -磁気活性化細胞選別(MACS)を使用してバルク。
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