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DOI :
10.3791/50210-v
May 17th, 2013
Chapters
0:05
Title
1:10
Colon Tumor Isolation and Cell Dissociation
2:52
Culture of Intestinal Tumor
3:41
Maintenance of Established Organoids
4:36
Storage and Recovery of Established Organoids
5:23
RNA Extraction, Protein Extraction and Immunohistochemistry
6:12
Results: Formation of Organoids
7:13
Conclusion
기본 생쥐 대장 종양 organoid을 설정하는 간단한 방법이 설명되어 있습니다. 이 방법은 대장 종양 세포가 생존하고 상피 정상적인 결장하지 않는 반면, 제한된 성장 인자를 포함하는 미디어 organoids로 성장하는 기능을 사용합니다.
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