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DOI :
10.3791/50222-v
February 19th, 2013
Chapters
0:05
Title
1:35
Preparation of Citrobacter rodentium Inoculum and Oral Gavage of Mice
3:03
Measuring Colonic Epithelial Barrier Permeability in C. rodentium-infected Mice
4:18
Measurement of Bacterial Load in Tissues of C. rodentium-infected Mice
6:06
Histological Assessment and Immunofluorescence Staining of Infected Colon Tissues
8:02
Results: Representative Overnight Culture Infection Data from a Frozen Glycerol Stock
10:14
Conclusion
シトロrodentium感染は腸内細菌感染症を勉強するだけでなく、マウスでは免疫応答や大腸炎をホストするための貴重なモデルを提供します。このプロトコルは、障壁の完全性、病原体負荷と病原体と宿主マウス感染性大腸炎への貢献を徹底特性評価を可能にする組織学的損傷の測定の概要を説明します。
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