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从手术标本的人类胰岛细胞激光显微切割的改进方法

DOI :

10.3791/50231-v

January 6th, 2013

January 6th, 2013

14,232 Views

1Molecular Diabetology, Paul Langerhans Institute Dresden, 2Department of GI-, Thoracic- and Vascular Surgery, University Hospital Carl Gustav Carus, University of Technology Dresden, 3Department of Endocrinology and Metabolism, Metabolic Unit University of Pisa, 4Labs DC0522, Lilly Corporate Center, 5Genomics, Faculty of Medicine Imperial College London, 6Vital-IT, SIB Swiss Institute of Bioinformatics, 7Clinical Biochemistry, Hannover Medical School, 8Cell Physiology and Metabolism, Medical School, University of Geneva, 9Department of Pathology, University Hospital Carl Gustav Carus, University of Technology Dresden, 10R&D DIAB Division / Translational Medicine, Sanofi-Aventis

激光显微切割是一种技术,使选定单元格的恢复微量的实质。在这里,我们描述了一个协议,收购手术标本用于转录组研究人类胰岛。我们的协议提高了内在的自发荧光的人类β细胞,从而有利于他们的收藏。

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