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DOI :
10.3791/50243-v
May 31st, 2013
Chapters
0:05
Title
1:40
RNA Folding
3:09
Chemical Modification of the RNA
4:33
Reverse Transcription
7:08
Preparation of Sequencing Ladder and Capillary Electrophoresis
9:55
ShapeFinder Software
11:15
Data Normalization
12:53
Conclusion
11:51
Results: RNA Structure Determination using RNA Structure Software
プライマー伸長により解析し、高スループット選択2 'ヒドロキシルアシル化(SHAPE)数百から一塩基の解像度で数千ヌクレオチドRNAの構造を決定するための技術が、逆転写、キャピラリー電気泳動および二次構造予測ソフトウェアをプロービングする新規化学物質を利用しています。
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