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液泡和胞质pH值的测量

DOI :

10.3791/50261-v

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April 19th, 2013

April 19th, 2013

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19,492 Views

Theodore T. Diakov¹, Maureen Tarsio¹, Patricia M. Kane¹

¹Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University

Chapters

可以测量活酵母液泡膜和细胞内pH值（ S。酵母）定位到特定的细胞区室的使用比例荧光染料的细胞。我们描述BCECF-AM，定位于液泡酵母，细胞内pH值与胞浆的比例的pH敏感GFP（酵母pHluorin所指示）测量空泡pH值的程序。

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