Name: designing a bio-responsive robot from dna origami
Uploaded: 2013-07-08T12:00:00
Description: Watch this Scientific Journal Video about Designing a Bio-responsive Robot from DNA Origami at JoVE.com

Forfatterne vil gerne takke S. Douglas for ekstremt værdifulde diskussioner og rådgivning, og alle medlemmer af Bachelet laboratorium til nyttige diskussioner og arbejde. Dette arbejde er støttet af tilskud fra Det Biovidenskabelige Fakultet og Institut for Nanoteknologi og Advanced Materials på Bar-Ilan University.

Robotten vil vi designe i dette papir imødekommer et protein P ved at lave en last C tilgængelig til at binde til receptorer på overfladen af en udvalgt målcelle. Robotten er vist i figur 1 C, kan være en receptor-blokerende stof,. En vækstfaktor etc., og en måde at kemisk knytte det til et DNA-oligonukleotid skal være til rådighed, der ikke ødelægger dens funktion. Robotten har to stater. Når inaktive, DNA porte på de to eksterne læber «er hybridiseret, og sør...

<ol>
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DNA-origami gør os til at fabrikere præcist definerede objekter med vilkårlige funktioner på nanoskala. Et vigtigt næste skridt ville være at integrere funktionen i disse mønstre. Mens mange applikationer og udfordringer kan løses med denne teknologi, der er en særlig interesse i opdigte terapeutiske og videnskabelige robotter fra DNA-origami, da disse udgør en naturlig miljø af DNA. DNA allerede grænseflader med molekylære maskineri i celler som en genetisk information lagermedium. Interessant, kan den fol...

<table border="1">
 <tbody>
 <tr>
 <td>Name of Reagent/Material</td>
 <td>Company</td>
 <td>Catalog Number</td>
 <td>Comments</td>
 </tr>
 <tr>
 <td>Autodesk Maya 2012</td>
 <td>Autodesk</td>
 <td></td>
 <td>A student/academic account needs to be created first (see platform-specific instructions in <a href="http://cadnano.org" target="_blank">http://cadnano.org</a>)</td>
 </tr>
 <tr>
 <td>caDNAno 2.0 (software)</td>
 <td>(Open source)</td>
 <td></td>
 <td>Software for the design of DNA origami structures <a href="http://cadnano.org/" target="_blank">http://cadnano.org</a></td>
 </tr>
 <tr>
 <td>Cando (webpage)</td>
 <td>(Open source)</td>
 <td></td>
 <td>Webpage running a simulator of DNA origami shapes <a href="http://cando-dna-origami.org" target="_blank">http://cando-dna-origami.org</a></td>
 </tr>
 </tbody>
</table>

designing a bio-responsive robot from dna origami

Nukleinsyrer er forbavsende alsidig. Ud over deres naturlige rolle som lagermedium for biologisk information 1, kan de anvendes i parallel computing 2,3, genkender og binder molekylære eller cellulære mål 4,5 katalyserer kemiske reaktioner 6,7, og generere beregnede reaktioner i en biologisk systemet 8,9. Vigtigt er det, kan nukleinsyrer programmeres til selv samle ind 2D-og 3D-strukturer 10-12, gør det muligt at integrere alle disse bemærkelsesværdige funktioner i en enkelt robot forbinder sensing af biologiske signaler til en forudindstillet reaktion for at udøve en ønsket effekt. Oprettelse figurer fra nukleinsyrer blev først foreslået af Seeman 13, og flere variationer over dette tema er siden blevet realiseret ved hjælp af forskellige teknikker 11,12,14,15. Men den mest betydningsfulde er måske den, der foreslås af Rothemund, betegnes scaffolded DNA-origami16.. I denne teknik er foldningen af en lang (&gt; 7.000 baser) enkeltstrenget DNA &#39;stillads »rettet til en ønsket form af hundredvis af korte komplementære strenge kaldes» hæfteklammer. Foldning udføres ved temperatur annealing rampe. Denne teknik blev succesfuldt demonstreret i skabelsen af ​​en bred vifte af 2D figurer med bemærkelsesværdig præcision og robusthed. DNA-origami blev senere udvidet til 3D samt 17,18. Den nuværende papirbaserede vil fokusere på caDNAno 2.0-software 19 udviklet af Douglas og kolleger. caDNAno er et robust, brugervenligt CAD-værktøj gør det muligt at udformningen af ​​2D-og 3D-DNA-origami figurer med alsidige funktioner. I designprocessen er afhængig af en systematisk og præcis abstraktion ordning for DNA strukturer, hvilket gør det relativt enkelt og effektivt. I dette papir viser vi udformningen af ​​en DNA-origami nanorobot der er for nylig blevet beskrevet 20. Denne robot er &quot;robot&quot; i den forstand, at det links sensing aktivering, for at udføre en opgave. Vi forklarer, hvordan forskellige sensing ordninger kan integreres i strukturen, og hvordan dette kan videresendes til en ønsket effekt. Endelig bruger vi Cando 21 at simulere de mekaniske egenskaber af den designede facon. Konceptet vi diskutere kan tilpasses til mange forskellige opgaver og indstillinger.

<html> Tal 1-25 er screenshots af caDNAno 2.0 interface viser designprocessen trin-for-trin. Tværsnittet af formen blev første gang beskrevet (figur 3), efterfulgt af automatisk tilsætning af stillads streng fragmenter og færdiggørelse af hele stilladset sti (figur 7). Staple tråde tilføjes automatisk (Figur 12), fordelt efter brugerdefinerede parametre (figur 14), og manuelt redigeret for at tilpasse hæfteklammer til den ønsked...

Watch this Scientific Journal Video about Designing a Bio-responsive Robot from DNA Origami at JoVE.com

Designing a Bio-responsive Robot from DNA Origami

DNA-origami er en stærk metode til fabrikere præcise nanoskala objekter ved at programmere den selv-samling af DNA-molekyler. Her beskriver vi, hvordan DNA-origami kan anvendes til at designe en robot robot kan registrere biologiske signaler og reagerer ved form flytte efterfølgende videresendes til en ønsket virkning.

Design af et Bio-responsiv Robot fra DNA Origami

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