Name: endotracheal intubation in mice via direct laryngoscopy using an otoscope
Uploaded: 2014-04-05T14:00:00
Description: Watch this Scientific Journal Video about Endotracheal Intubation in Mice via Direct Laryngoscopy Using an Otoscope at JoVE.com

从退伍军人事务部的优点格兰特和来自美国国立卫生研究院的NHLBI一个T32-HL098062资金支持这项工作。我们要衷心感谢兰多夫H.黑廷斯，医学博士，博士的意见以及建议和支持弗吉尼亚州圣迭戈医疗系统的兽医医疗单位。

1。动物的制备<ol><li>获得小鼠早于8周，超过20克（小老鼠可以由专家插管）。 </li><li>麻醉<ol><li>注入老鼠用20毫克/千克氯胺酮和赛拉嗪腹膜内作为麻醉前，每个。 （该剂量是不足以完全麻醉的小鼠，但有利于安全转移，气管插管后，机械通气。）但是，调整该剂量可根据与体制兽医会诊麻醉反应是必要的。） </li><li>麻醉诱导小鼠与3.5％异氟醚/氧气感应室为90-120秒。 </li><li>仔细观察呼吸频率。...

<ol>
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Lung Res. 36, 25-30 (2010).</li></ol>

在本报告中，我们描述一个简单的，可靠的技术来插管小鼠即非创伤性，并且可以在相同的动物反复使用。这种技术可以完成简单的实验室，或者被一个个​​小数目，可以购买医疗设备。直接喉镜的技术中，最初报告由Hastings和同事4，也可用于各种用途，但主要还是以准确递送试验物质到下呼吸道。我们发现这种技术，因为它的易用性，速度，简单，可靠，费用低，和潜在的重复测量?...

该实验室鼠标已经取代几乎所有的物种生物学和病理学的主要哺乳动物模型。该实验室的鼠标是已明确且全面证明是有价值的人类疾病的模型，并证明无价在我们人类生物学和疾病的认识的进步最小的哺乳动物。短期妊娠时间和大大降低了成本允许空和转基因小鼠的发展和研究，在生物医学研究的一个司空见惯的工具。然而，一般的实验室小鼠（20-25克）的大小限制了他们的研究在生理学或手术为基础的研究，因此，一些研究者研究大哺乳动物。妨碍利用老鼠在这些研究中是与插管技术，这将允许在深度麻醉下生理测量或广泛的外科手术中遇到的困难。气管切开1已被用作标准特chnique因为更容易利用这项技术并适度的技能需要的，而不是插管。然而，气管切开，不利于慢性或恢复手术研究;因此，它被限制在急性实验。气管造口术，也可以在研究一个混杂变量，其中炎症或敏感的生理反射是很重要的。 我们的实验室已尝试大部分由其他研究者描述的技术，并发现它们不足以用于各种原因。气管切开术是太痛苦并诱导出血及气道炎症。更成问题的是，它不能切实重复。许多需要适度的设备投资相对非侵入性的技术是不是足够可靠。其他技术需要昂贵的设备，是很难，如果不知道该设备将工作在一个特定的应用程序来证明。因此，我们试图开发一种非创伤性技术，它不需要更多的T汉适度的投资在专门的设备，可以迅速完成，并且可靠，可以重复在慢性模型中，并且可以在大量动物中使用。在这里，我们报告这样的技术。

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			<td height="17" style="height:17px;width:411px;">Operating Otoscope Head</td>
			<td style="width:144px;">Welch Allyn</td>
			<td align="right" style="width:83px;">21700</td>
			<td style="width:69px;">&#160;$188.98</td>
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			<td height="17" style="height:17px;">Otoscope Handle</td>
			<td>Welch Allyn</td>
			<td align="right">71000</td>
			<td>&#160;$112.20</td>
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			<td>Welch Allyn</td>
			<td align="right">22002</td>
			<td>&#160;$ 3.98</td>
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			<td>18-779</td>
			<td>&#160;$107.18</td>
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			<td>Summit Medical</td>
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			<td>SCIREQ</td>
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			<td height="17" style="height:17px;">Supplies</td>
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			<td height="17" style="height:17px;">Intravenous catheter (20 ga x 1 inch)</td>
			<td>BD</td>
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			<td>&#160;$&#160; 9.62</td>
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			<td height="17" style="height:17px;">Polyethylene tubing (PE10) (0.011 inch I.D., 0.024 inch O.D.) 100 ft</td>
			<td>Intramedic, Clay-Adams</td>
			<td align="right">427401</td>
			<td>&#160;$115.70</td>
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			<td height="17" style="height:17px;">Ketamine 100 mg/ml (10 ml bottle)</td>
			<td>Butler</td>
			<td align="right">23061</td>
			<td>&#160;$10.00</td>
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			<td height="17" style="height:17px;">Xylazine (100 ml bottle)</td>
			<td>Vedco</td>
			<td align="right">24105</td>
			<td>&#160;$20.00</td>
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			<td height="17" style="height:17px;">Isoflurane (250 ml bottle)</td>
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			<td></td>
			<td>&#160;$15.00</td>
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			<td height="17" style="height:17px;">vecuronium bromide 10 mg/10 ml</td>
			<td>Pfizer</td>
			<td>NDC 0069-0094-01</td>
			<td align="right">$15.00</td>
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endotracheal intubation in mice via direct laryngoscopy using an otoscope

小鼠，无论是野生型和转基因，是主要的哺乳动物模型在生物医学研究当前。插管和机械通气是必要的，需要深麻醉下或肺功能测量整个手术的动物实验。气管切开术一直是插​​管气道在这些小鼠，使机械通气的标准。经口气管插管有报道由于大量的技术困难或高度专业化的和昂贵的设备的要求，但并没有在许多研究中得到成功应用。此处我们报告直接喉镜的使用配有2.0mm的窥器耳镜，并使用20 G静脉导管为气管内管的技术。我们已经用这种技术广泛和可靠的插管和小鼠进行肺功能的准确评估。这种技术已被证明安全的，有经验的人员基本上没有动物的损失。此外，这种技术可用于小鼠的慢性模型反复研究。

插管与上述技术是可靠和快速。气管内管的适当位置是最容易通过的水下呼气肢观察呼出气鼓泡验证了呼吸机回路（通常在PEEP陷阱）和上气道压力跟踪（ 图1）负偏差的。上气道压力跟踪的负偏差是最可靠的。其他人在使用静脉注射管连接到呼气呼吸机回路2的小液滴的运动。虽然我们没有使用这种技术广泛应用，它应该足以验证管的位置。一个有经验的操作者可以使用这个?...

Watch this Scientific Journal Video about Endotracheal Intubation in Mice via Direct Laryngoscopy Using an Otoscope at JoVE.com

Endotracheal Intubation in Mice via Direct Laryngoscopy Using an Otoscope

我们已经开发出用耳镜用2.0mm的窥器通过直接喉镜气管插管在小鼠一个简单，可靠，且相对廉价的方法。这种技术是无创伤和可用于在慢性实验重复测量。我们发现它优于气管造口术或先前报道非手术技术。

气管插管小鼠<em&gt;通过</em&gt;直接喉镜使用耳镜

Mice, both wildtype and transgenic, are the principal mammalian model in biomedical research currently. Intubation and mechanical ventilation are ...

Research

JoVE Journal

Medicine

Endotracheal Intubation in Mice via Direct Laryngoscopy Using an Otoscope

Guidelines for Elective Pediatric Fiberoptic Intubation

选修儿科光纤插管指南

Fiberoptic intubation in pediatric patients is often required especially in difficult airways of syndromic patients i.e. Pierre Robin Syndrome. Small ...

科研

医学

Using Quantitative Real-time PCR to Determine Donor Cell Engraftment in a Competitive Murine Bone Marrow Transplantation Model

采用实时定量PCR检测供体细胞植入一个具有竞争力的小鼠骨髓移植模型

Murine bone marrow transplantation models provide an important tool in measuring hematopoietic stem cell (HSC) functions and determining genes/molecules ...

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一个简单的方法小鼠肺插管

A  simple procedure to intubate mice for pulmonary function measurements would  have several advantages in longitudinal studies with limited numbers or  ...

Heterotopic Mucosal Engrafting Procedure for Direct Drug Delivery to the Brain in Mice

异位黏膜移植程序直接给药到小鼠的大脑

Delivery of therapeutics into the brain is impeded by the presence of the blood-brain barrier (BBB) which restricts the passage of polar and high ...

An Improved Method for Rapid Intubation of the Trachea in Mice

在小鼠气管插管快速的改进方法

Despite some anatomical and physiological differences, mouse models continue to be an essential tool for studying human lung disease. Bleomycin toxicity ...

Uncontrolled Hemorrhagic Shock Modeled via Liver Laceration in Mice with Real Time Hemodynamic Monitoring

通过实时血液动力学监测在小鼠中通过肝脏浸润建立的不受控制的出血性休克

Uncontrolled hemorrhage is an important cause of preventable deaths among trauma patients. We have developed a murine model of uncontrolled hemorrhage via ...

Middle Cerebral Artery Occlusion Allowing Reperfusion via Common Carotid Artery Repair in Mice

颅内动脉闭塞允许经普通颈动脉修复再灌注的研究

The ischemic stroke is a major cause of adult long-term disability and death worldwide. The current treatments available are limited, with only tissue ...

Inducing Acute Lung Injury in Mice by Direct Intratracheal Lipopolysaccharide Instillation

通过直接内切内脂多糖灌输诱导小鼠急性肺损伤

Airway administration of lipopolysaccharide (LPS) is a common way to study pulmonary inflammation and acute lung injury (ALI) in small animal models. ...

Repeated Orotracheal Intubation in Mice

小鼠重复的奥内切管插管

The literature describes several methods for mouse intubation that either require visualization of the glottis through the oral cavity or incision in the ...

Blind Endotracheal Intubation in Neonatal Rabbits

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The newborn rabbit is a useful animal model for various pathologies and procedures. Airway management of the rabbit is complex due to its anatomical ...