Name: graphene coatings for biomedical implants
Uploaded: 2013-03-01T13:00:00
Description: Watch this Scientific Journal Video about Graphene Coatings for Biomedical Implants at JoVE.com

1. Graphene-coating af NiTi <ol><li> De graphene prøver anvendt i dette studie blev dyrket på kobber (Cu) substrater ved hjælp af kemisk dampaflejring teknik, og efterfølgende overført til 4,5 mm 2 NiTi substrater. </li><li> Cu folier (1 cm x 1 cm) blev anbragt i en 1 in kvartsrør ovn og opvarmet til 1000 ° C i nærværelse af 50 sccm af H2 og 450 sccm med Ar. </li><li> Next, methan (1 og 4 sccm) indført i ovnen ved forskellige strømningshastigheder for 20-30 min. Prøverne blev endelig ...

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Biokompatibilitet og cytotoksicitet: The kemisk dampudfældning (CVD) metode gav polykrystallinske graphene prøver at efterlignede Cu krystalkorn som vist i figur 1a. Vi anvendte Raman spektroskopi for at bekræfte tilstedeværelsen af monolaget (få lag) graphene på 1 sccm (4 sccm) prøver (se figur 1b). Det er klart, 1 SCCM (4 sccm) prøver udviser intens (relativt svagere) G &#39;band indikerer monolag (få lag) graphene. 1c viser en atomic force mikroskop...

De seneste tre årtier har været vidne til opdagelsen af ​​nye materialer-baserede terapier og indretninger til sygdomsbehandlinger og diagnostik. Nye legering materialer såsom nitinol (NiTi) og rustfrit stål anvendes ofte i biomedicinsk implantat fremstilling på grund af deres overlegne mekaniske egenskaber. 1-3 imidlertid talrige udfordringer på grund af eksogent materiale cytotoksicitet, bio-og hæmo-kompatibilitet. Den metalliske natur af disse legeringer resulterer i dårlig bio-og hemocompatibility grund metal udvaskning, mangel på celleadhæsion, proliferation og thrombose, når det kommer i kontakt med strømmende blod (såsom katetre, blodkartransplantater, vaskulære stents, kunstige hjerteklapper etc.). 1, 4, 5 Interaktionen af proteiner eller levende celler med implantatoverfladen kan føre til en kraftig immunologisk reaktion og den efterfølgende kaskade af biokemiske reaktioner kan påvirke enhedens funktionalitet. Derfor er det pertinent for at opnå kontrol over samspillet mellem biomedicinske implantater og dens omgivende biologisk miljø. Overflademodifikation anvendes ofte til at reducere eller forebygge de negative fysiologiske respons hidrørende fra implantatmaterialet. En ideel overfladebelægning forventes at have høj adhæsionsstyrke, kemisk stabilitet, høj glathed, og god hæmo-og bioforligelighed. Tidligere har mange materialer, herunder diamant-lignende carbon (DLC), SiC, TiN, TiO 2 og mange polymermaterialer blevet testet som bio-kompatible implantater overfladebelægninger. 1, 6-23 Men disse materialer er stadig ikke i stand til at opfylde alle de funktionelle kriterier for en egnet implantat overfladebelægning. Opdagelsen af atom tykt lag af sp 2 kulstof, kendt som graphene, har åbnet dørene for udvikling af nye multifunktionelle materialer. Graphene forventes at være en ideel kandidat til implantat overfladebelægning, da deter kemisk inert, atomically glat og meget holdbar. I dette brev undersøger vi levedygtigheden af ​​graphene som en overfladebelægning til biomedicinske implantater. Vore undersøgelser viser, at graphene overtrukne nitinol (Gr-NiTi) opfylder alle de funktionelle kriterier, og yderligere støtter fremragende glat muskulatur og endotelcellevækst fører til bedre celleproliferation. Vi finder endvidere den serumalbumin adsorption på Gr-NiTi er højere end fibrinogen. Vigtigt er, (i) vore detaljerede spektroskopiske målinger bekræftede manglen på ladningsoverførsel mellem graphene og fibrinogen antyder, at graphene coating inhiberer blodpladeaktivering af implantater, (ii) graphene overtræk udviser ikke nogen signifikant in vitro-toksicitet af endothel-og glatte muskelceller cellelinier bekræfter deres biokompatibilitet, og (iii) graphene belægninger er kemisk inerte, hårde og vandtætte i strømmende blod miljø. Disse hæmo-og biokompatible egenskaber samt høj strength, kemisk stabilitet og holdbarhed, gør graphene belægninger som en ideel overfladebelægning.

<table border="1"><tbody><tr><td></td><td></td><td></td><td> Reagens </td></tr><tr><td> Dulbeccos modificerede Eagle-medium </td><td> ATCC </td><td> 30-2002 </td><td></td></tr><tr><td> Thiazolyl blå tetrazoliumbromid </td><td> Sigma-Aldrich </td><td> M2128 </td><td></td></tr><tr><td> CellTiter 96 Aqueous One opløsning celleproliferationsassay (MTS) </td><td> Promega </td><td> G3582 </td><td></td></tr><tr><td> Dimethylsulfoxid </td><td> Sigma-Aldrich </td><td> D8418 </td><td></td></tr><tr><td> 36,5% formaldehyd </td><td> Sigma-Aldrich </td><td> F8775 </td><td></td></tr><tr><td> Triton X-100 </td><td> Sigma-Aldrich </td><td> T8787 </td><td></td></tr><tr><td> Alexafluor 488 phalloidin </td><td> Life Technologies </td><td> A12379 </td><td></td></tr><tr><td> Vectashield monteringsmedium med DAPJeg </td><td> Vector Laboratories </td><td> H-1200 </td><td></td></tr><tr><td> Humant serumalbumin </td><td> Sigma-Aldrich </td><td> A9511 </td><td></td></tr><tr><td> Humanfibrinogen </td><td></td><td></td><td></td></tr><tr><td> Tris / Glycine / SDS </td><td> Bio-Rad </td><td> 161-0732 </td><td></td></tr><tr><td> Ready Gel Tris-HCI Gel </td><td> Bio-Rad </td><td> 161-1158 </td><td></td></tr><tr><td> Eddikesyre </td><td> Sigma-Aldrich </td><td> 45.726 </td><td></td></tr><tr><td> SYPRO Rød </td><td> Life Technologies </td><td> S-6653 </td><td></td></tr><tr><td> Protein lav BCA-assay </td><td> Lamda Biotech </td><td> G1003 </td><td></td></tr><tr><td> Precision Plus Protein Kaleidoscope Standard </td><td> Bio-Rad </td><td> 161-0375 </td><td></td></tr><tr><td> Immun-Blot PVDF-membran </td><td> Bio-Rad </td><td> 162-0177 </td><td></td></tr><tr><td> Blotting kvalitet blokker ikke-fedtholdig tørmælk </td><td> Bio-Rad </td><td> 170-6404XTU </td><td></td></tr><tr><td> Anti-actin-antistof fremstillet i kaniner </td><td> Sigma-Aldrich </td><td> A2066 </td><td></td></tr><tr><td> BM Chemiluminescence Western Blotting Kit (muse / kanin) </td><td> Roche Applied Science </td><td> 11520709001 </td><td></td></tr><tr><td> RIPA-buffer </td><td> Sigma-Aldrich </td><td> R0278 </td><td></td></tr><tr><td> NiTi (51% Ni, 49% Ti) </td><td> Alfa-Aesar </td><td> 44.953 </td><td></td></tr><tr><td></td><td></td><td></td><td> Udstyr </td></tr><tr><td> Horiba JobinYvon </td><td> Raman-spektrometer </td><td> Dilor XY 98 </td><td></td></tr><tr><td> Nikon </td><td> Konfokalt mikroskop </td><td> Eclipse TI mikroskop </td><td></td></tr&gt; <tr><td> Thermoscientific </td><td> Pladeaflæser </td><td></td><td></td></tr><tr><td> Bio-Rad </td><td> Strømforsyning </td><td> 164-5050 </td><td> PowerPac basic strømforsyning </td></tr><tr><td> Bio-Rad </td><td> Electrophoresis celle </td><td> 165-8004 </td><td> Mini-PROTEAN tetra celle </td></tr><tr><td> Bio-Rad </td><td> Gel holder kassette </td><td> 170-3931 </td><td> Mini gel holder kassette </td></tr></tbody></table>

graphene coatings for biomedical implants

Atomically glat graphene som en overfladebelægning har potentiale til at forbedre implantat egenskaber. Dette viser en fremgangsmåde til belægning af nitinol legeringer med nanometer tykke lag af graphene til anvendelse som en stent materiale. Graphene blev dyrket på kobber substrater via kemisk dampafsætning og derefter overført til nitinol substrater. For at forstå, hvordan graphene coating kunne ændre biologisk respons blev cellelevedygtighed af rotte-aorta-endothelceller og rotte aorta glatmuskelceller undersøgt. Desuden blev virkningen af ​​graphene-overtræk på celleadhæsion og morfologi undersøgt med fluorescerende konfokal mikroskopi. Celler blev farvet for actin og kerner, og der var tydelige forskelle mellem uberørte nitinol prøver sammenlignet med graphene-coatede prøver. I alt actin ekspression fra rotte aorta glatmuskelceller blev fundet ved hjælp af western blot. Proteinadsorption karakteristika, en indikator for potentiel thrombogenicitet, were bestemt for serum albumin og fibrinogen med gelelektroforese. Desuden blev overførsel af ladning fra fibrinogen til substratet udledt ved hjælp af Raman-spektroskopi. Det konstateredes, at graphene belægning på nitinol substrater opfyldt de funktionelle krav til en stent materiale og forbedret biologiske respons sammenlignet med uovertrukne nitinol. Således graphene-belagt nitinol er en mulig kandidat til en stent materiale.

<img src="/files/ftp_upload/50276/50276fig1.jpg" alt="Figur 1"/> . Figur 1 a) CVD dyrket polykrystallinske graphene på Cu folier efterligner metal krystalkorn (skala bar: 10 pm). b) Raman-spektrum af 1 sccm (4 sccm) graphene viser intens (relativt svagere) G &#39;band indikerer monolag (få lag) arten af ​​som fremstillet graphene. c) AFM billede af graphene overføres til NiTi viser en ruhed på ~ 5 nm. Scale bar = 500 nm. <p class="jove_content" fo:k...

Watch this Scientific Journal Video about Graphene Coatings for Biomedical Implants at JoVE.com

Graphene Coatings for Biomedical Implants

Graphene giver potentiale som et coatingmateriale til biomedicinske implantater. I denne undersøgelse har vi demonstrere en metode til belægning af nitinol legeringer med nanometer tykke lag af graphene og bestemme, hvordan graphene kan påvirke implantatet respons.

Graphene Maling til biomedicinske implantater

Atomically smooth graphene as a surface coating has potential to improve implant properties. This demonstrates a method for coating nitinol alloys with ...

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Rapid Mix Preparation of Bioinspired Nanoscale Hydroxyapatite for Biomedical Applications

Hurtig Mix Udarbejdelse af bioinspirerede Nanoscale hydroxyapatit for Biomedical Applications

Hydroxyapatite (HA) has been widely used as a medical ceramic due to its good biocompatibility and osteoconductivity. Recently there has been interest ...

Microhoneycomb Monoliths Prepared by the Unidirectional Freeze-drying of Cellulose Nanofiber Based Sols: Method and Extensions

Microhoneycomb Monoliter udarbejdet af den ensrettede frysetørring af Cellulose Nanofiber baseret Sols: metode og udvidelser

Monolithic honeycomb structures have been attractive to multidisciplinary fields due to their high strength-to-weight ratio. Particularly, microhoneycomb ...

Manufacturing of a Nafion-coated, Reduced Graphene Oxide/Polyaniline Chemiresistive Sensor to Monitor pH in Real-time During Microbial Fermentation

Fremstilling af en Nafion-belagt, reduceret Graphene oxid/Polyaniline Chemiresistive Sensor til skærm pH i Real-time under mikrobiel gæring

Here, we report the engineering of a solid-state micro pH sensor based on polyaniline-functionalized, electrochemically reduced graphene oxide (ERGO-PA). ...

Environmental Dynamic Mechanical Analysis to Predict the Softening Behavior of Neural Implants

Miljømæssige dynamisk mekanisk analyse til at forudsige den blødgørende opførsel af neurale implantater

When using dynamically softening substrates for neural implants, it is important to have a reliable in vitro method to characterize the softening behavior ...

Mitigation of Blood Borne Cell Attachment to Metal Implants through CD47-Derived Peptide Immobilization

Afbødning af blodbårne celle vedhæftet fil til metal implantater gennem CD47-Afledt peptid immobilisering

The key complications associated with bare metal stents and drug eluting stents are in-stent restenosis and late stent thrombosis, respectively. Thus, ...