Name: graphene coatings for biomedical implants
Uploaded: 2013-03-01T13:00:00
Description: Watch this Scientific Journal Video about Graphene Coatings for Biomedical Implants at JoVE.com

1. Graphene-belegg av NiTi <ol><li> Graphene ble brukt i denne studien ble dyrket på kobber (Cu) underlag ved hjelp av kjemiske damp avsetning teknikk, og senere overført til 4,5 mm 2 NiTi underlag. </li><li> Cu foliene (1 cm x 1 cm) ble plassert i en 1 igjen kvarts rørovn og oppvarmet til 1000 ° C i nærvær av 50 SCCM av H 2 og 450 SCCM Ar. </li><li> Neste, metan (1 og 4 SCCM) ble introdusert inn i ovnen på forskjellige strømningsrater for 20-30 min. Prøvene ble endelig avkjølt til romt...

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Biokompatibilitet og cytotoksisitet: Den kjemiske dampavsetning (CVD)-metoden ga polykrystallinske graphene prøver som etterlignet Cu krystall korn, som vist i Figur 1a. Vi ansatt Raman spektroskopi for å bekrefte tilstedeværelse av monolayer (noen lag) graphene på en SCCM (4 SCCM) prøver (se Figur 1b). Tydelig, en SCCM (4 SCCM) prøver viser intense (relativt svakere) G &#39;bandet indikerer monolayer (noen lag) graphene. Figur 1c viser en atomic force mi...

De siste tre tiårene har vært vitne oppdagelsen av nye materialer-baserte terapier og utstyr for sykdom behandlinger og diagnostikk. Nye legering materialer som Nitinol (NiTi) og rustfritt stål er ofte brukt i biomedisinsk implantat produksjon på grunn av sin overlegne mekaniske egenskaper. 1-3 Men mange utfordringer gjenstår på grunn eksogen materiale cytotoksisitet, bio-og hemo-kompatibilitet. Den metalliske natur av disse legeringer resulterer i dårlig bio-og hemocompatibility grunnet metall utvasking, mangel av celleadhesjon, spredning, og trombose når det kommer i kontakt med strømmende blod (slik som katetere, blodkar grafts, vaskulære stenter, kunstige hjerteklaffer el.). 1, 4, 5 Samspillet proteiner eller levende celler med implantatet overflate kan føre til en sterk immunologisk respons og den påfølgende kaskade av biokjemiske reaksjoner kan påvirke enhetens funksjonalitet. Derfor er det pertinent for å oppnå kontroll over samspillet mellom biomedisinske implantater og dens omkringliggende biologiske miljø. Overflatemodifisering blir ofte brukt til å redusere eller hindre uønskede fysiologiske responsen stammer fra implantatmaterialet. En ideell overflatebelegg er forventet å ha høy vedheft styrke, kjemisk inertness, høy jevnhet og god hemo-og biokompatibilitet. Tidligere har en rekke materialer, inkludert diamant-lignende karbon (DLC), SiC, TiN, TiO 2 og mange polymermaterialer blitt testet som bio-kompatible implantat overflatebehandlinger. 1, 6-23 Men disse materialene er fortsatt i stand til å møte alle de funksjonelle kriterier for et egnet implantat overflatebelegg. Oppdagelsen av atom tykke lag av sp 2 karbon, kjent som graphene, har åpnet dører for utvikling av nye multifunksjonelle materialer. Graphene er forventet å være en ideell kandidat for implantat overflatebelegg siden deter kjemisk inert, atomically jevn og meget slitesterk. I dette brevet, undersøker vi levedyktigheten til graphene som en overflate belegg for biomedisinsk implantater. Våre studier viser at graphene belagt Nitinol (Gr-NiTi) oppfyller alle funksjonelle kriterier, og i tillegg støtter utmerket glatt muskulatur og endotelcelle vekst fører til bedre celleproliferasjon. Vi finner også at serum albumin adsorpsjon på Gr-NiTi er høyere enn fibrinogen. Viktigere, (i) våre detaljerte spektroskopiske målinger bekreftet mangelen omkostninger overføring mellom graphene og fibrinogen tyder at graphene belegg hemmer plateaktivering av implantater, (ii) graphene belegg ikke oppviser noen betydelig in vitro giftighet for endotelceller og glatte muskel cellelinjer bekrefter deres biokompatibilitet, og (iii) graphene belegg er kjemisk inert, holdbare og ugjennomtrengelig i strømmende blod miljø. Disse hemo-og biokompatible egenskapene, sammen med høy strength, kjemisk inertness og holdbarhet, gjør graphene belegg som en ideell overflate belegg.

<table border="1"><tbody><tr><td></td><td></td><td></td><td> Reagens </td></tr><tr><td> Dulbeccos modifiserte Eagle Medium </td><td> ATCC </td><td> 30-2002 </td><td></td></tr><tr><td> Tiazolyl blå tetrazolium bromide </td><td> Sigma-Aldrich </td><td> M2128 </td><td></td></tr><tr><td> CellTiter 96 Vandig En løsning celleproliferasjon analysen (MTS) </td><td> Promega </td><td> G3582 </td><td></td></tr><tr><td> Dimetylsulfoksid </td><td> Sigma-Aldrich </td><td> D8418 </td><td></td></tr><tr><td> 36,5% formaldehyd </td><td> Sigma-Aldrich </td><td> F8775 </td><td></td></tr><tr><td> Triton X-100 </td><td> Sigma-Aldrich </td><td> T8787 </td><td></td></tr><tr><td> Alexafluor 488 phalloidin </td><td> Life Technologies </td><td> A12379 </td><td></td></tr><tr><td> VECTASHIELD montering medium med DAPJeg </td><td> Vector Laboratories </td><td> H-1200 </td><td></td></tr><tr><td> Humant serumalbumin </td><td> Sigma-Aldrich </td><td> A9511 </td><td></td></tr><tr><td> Humant fibrinogen </td><td></td><td></td><td></td></tr><tr><td> Tris / Glycine / SDS </td><td> Bio-Rad </td><td> 161-0732 </td><td></td></tr><tr><td> Klar Gel Tris-HCl Gel </td><td> Bio-Rad </td><td> 161-1158 </td><td></td></tr><tr><td> Eddiksyre </td><td> Sigma-Aldrich </td><td> 45726 </td><td></td></tr><tr><td> SYPRO Red </td><td> Life Technologies </td><td> S-6653 </td><td></td></tr><tr><td> Protein lav BCA analysen </td><td> Lamda Biotech </td><td> G1003 </td><td></td></tr><tr><td> Presisjon Plus Protein Kaleidoscope Standard </td><td> Bio-Rad </td><td> 161-0375 </td><td></td></tr><tr><td> Immun-Blot PVDF membran </td><td> Bio-Rad </td><td> 162-0177 </td><td></td></tr><tr><td> Blotting grade blocker fettfri tørrmelk </td><td> Bio-Rad </td><td> 170-6404XTU </td><td></td></tr><tr><td> Anti-aktin antistoff produsert i kanin </td><td> Sigma-Aldrich </td><td> A2066 </td><td></td></tr><tr><td> BM kjemiluminescens Western Blotting kit (mus / kanin) </td><td> Roche Applied Science </td><td> 11520709001 </td><td></td></tr><tr><td> RIPA buffer </td><td> Sigma-Aldrich </td><td> R0278 </td><td></td></tr><tr><td> NiTi (51% Ni, 49% Ti) </td><td> Alfa-Aesar </td><td> 44953 </td><td></td></tr><tr><td></td><td></td><td></td><td> Utstyr </td></tr><tr><td> HORIBA JobinYvon </td><td> Raman spektrometer </td><td> Dilor XY 98 </td><td></td></tr><tr><td> Nikon </td><td> Confocal mikroskop </td><td> Eclipse TI mikroskop </td><td></td></tr&gt; <tr><td> Thermoscientific </td><td> Plateavleserens </td><td></td><td></td></tr><tr><td> Bio-Rad </td><td> Strømforsyning </td><td> 164-5050 </td><td> PowerPac grunnleggende strømforsyning </td></tr><tr><td> Bio-Rad </td><td> Elektroforese celle </td><td> 165-8004 </td><td> Mini-protean tetra celle </td></tr><tr><td> Bio-Rad </td><td> Gel holder kassett </td><td> 170-3931 </td><td> Mini gel holder kassett </td></tr></tbody></table>

graphene coatings for biomedical implants

Atomically glatt graphene som et overflatebelegg har potensial til å forbedre implantat egenskaper. Dette demonstrerer en fremgangsmåte for belegging av Nitinol legeringer med nanometer tykt lag av graphene for applikasjoner som en stent materiale. Graphene ble dyrket på kobber underlag via kjemiske damp avsetning og deretter overført til Nitinol underlag. For å forstå hvordan graphene belegget kunne endre biologisk respons, ble cellelevedyktighet rotte aorta endotelceller og rotte aorta glatte muskelceller undersøkt. Videre ble effekten av graphene-beleggene på celleadhesjon og morfologi undersøkt med fluorescerende konfokalmikroskopi. Celler ble farget for aktin og atomkjerner, og det var merkbare forskjeller mellom uberørte Nitinol prøver sammenlignet med graphene-belagte prøver. Total aktin uttrykk fra rotte aorta glatte muskelceller ble funnet ved hjelp av Western blot. Protein adsorpsjon egenskaper, en indikator for potensielle thrombogenicity, wERE fastsettes for serumalbumin og fibrinogen med gelelektroforese. Videre ble overføring av belastning fra fibrinogen til substrat utledet med Raman spektroskopi. Det ble funnet at graphene belegg på Nitinol substrater møtte de funksjonelle krav til en stent materiale og forbedret biologisk respons sammenlignet ubelagte Nitinol. Dermed er graphene-belagt Nitinol en levedyktig kandidat for en stent materiale.

<img src="/files/ftp_upload/50276/50276fig1.jpg" alt="Figur 1"/> . Figur 1 a) CVD vokst polycrystalline graphene på Cu folie etterligner metall krystall korn (skala bar: 10 mikrometer). b) Raman spektrum av en SCCM (4 SCCM) graphene viser intense (relativt svakere) G &#39;bandet indikerer monolayer (noen lag) natur som forberedt graphene. c) AFM bilde av graphene overført til NiTi viser en ruhet ~ 5 nm. Skala bar = 500 nm. <p class="jove_content" fo:keep-...

Watch this Scientific Journal Video about Graphene Coatings for Biomedical Implants at JoVE.com

Graphene Coatings for Biomedical Implants

Graphene gir potensiale som et beleggmateriale for biomedisinske implantater. I denne studien viser vi en metode for belegg Nitinol legeringer med nanometer tykke lag av graphene og bestemme hvordan graphene kan påvirke implantat respons.

Graphene Belegg for Biomedical Implantater

Atomically smooth graphene as a surface coating has potential to improve implant properties. This demonstrates a method for coating nitinol alloys with ...

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Hydroxyapatite (HA) has been widely used as a medical ceramic due to its good biocompatibility and osteoconductivity. Recently there has been interest ...

Microhoneycomb Monoliths Prepared by the Unidirectional Freeze-drying of Cellulose Nanofiber Based Sols: Method and Extensions

Microhoneycomb Monoliths utarbeidet av den enveis Frysetørring av Cellulose Nanofiber basert Sols: metoden og utvidelser

Monolithic honeycomb structures have been attractive to multidisciplinary fields due to their high strength-to-weight ratio. Particularly, microhoneycomb ...

Manufacturing of a Nafion-coated, Reduced Graphene Oxide/Polyaniline Chemiresistive Sensor to Monitor pH in Real-time During Microbial Fermentation

Produksjon av en Nafion-belagt, redusert Grafén oksid/Polyaniline Chemiresistive Sensor til skjermen pH i sanntid under mikrobiell gjæring

Here, we report the engineering of a solid-state micro pH sensor based on polyaniline-functionalized, electrochemically reduced graphene oxide (ERGO-PA). ...

Environmental Dynamic Mechanical Analysis to Predict the Softening Behavior of Neural Implants

Miljømessige dynamisk mekanisk analyse å forutsi mykgjørende virkemåten av nevrale implantater

When using dynamically softening substrates for neural implants, it is important to have a reliable in vitro method to characterize the softening behavior ...

Mitigation of Blood Borne Cell Attachment to Metal Implants through CD47-Derived Peptide Immobilization

Reduksjon av blodbårne celletilbehør til metallimplantater gjennom CD47-avledet peptidimmobilisering

The key complications associated with bare metal stents and drug eluting stents are in-stent restenosis and late stent thrombosis, respectively. Thus, ...