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DOI :
10.3791/50295-v
•
10:57 min
March 25th, 2013
Chapters
0:05
Title
1:15
Chick Embryo Preparation
3:33
Embryo Slice Preparation
5:48
Culturing Embryo Slices
6:22
Sectioning the Slices for Immunostaining
7:49
Results: Development of the Lateral Motor Column in Chicken Embryo Slices
10:35
Conclusion
切片文化促进胚胎发育的基因和药理学的扰动操作。然而,文化条件必须确保可以继续正常发展的片内减少环境。我们说明了一个协议,有利于脊髓发育正常进行至少24小时。
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