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DOI :
10.3791/50300-v
April 2nd, 2013
Chapters
0:05
Title
2:13
Preparation of Legionella pneumophila Cultures for Priming Stage Infections
4:14
Priming Stage Infection Using Acanthamoeba castellanii
7:13
Processing of Bacteria for Target Cell Stage Infection after the Priming Stage Infection
9:46
Experimental Analysis
11:14
CFU Plating
11:51
Representative Infection Results
13:23
Conclusion
この手法は、収穫する方法を提供するものであり、哺乳動物細胞の感染に先立って自然原虫の宿主細胞にあらかじめ栽培されている細菌性病原体の細胞内増殖を正常化し、定量化する。このメソッドは、プライミング段階のための宿主細胞、ならびに標的細胞の種類の多種多様に対応するように変更することができます。
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