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DOI :
10.3791/50307-v
•
10:41 min
July 24th, 2014
Chapters
0:05
Title
1:39
Dissection Preparation
3:15
Presomitic Mesoderm Dissection and Dispersal
6:40
Imaging Dispersed Presomitic Mesodermal Cells
7:58
Image Processing of Acquired Time-lapse Movies
8:58
Representative Time-lapse Imaging Results
10:00
Conclusion
体节形成是有节奏的发育过程,从而在空间型态脊椎动物胚胎的体轴。以前,我们开发了使用荧光记者观察环状基因,推动这一进程的转基因斑马鱼线。在这里,我们培养从这些线和图像的振荡时间的推移在体外分散细胞。
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