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DOI :
10.3791/50308-v
•
13:32 min
April 10th, 2013
Chapters
0:05
Title
2:00
Sectioning and Staining of Rat Brain for LCM
4:28
LCM of Swaths of Enriched Populations of Hippocampal Neurons
8:41
LCM of Single FJ+ Neurons in the Hippocampus
10:42
Results: Laser Capture Microdissection of Individual and Swaths of Hippocampal Neurons
12:51
Conclusion
我们描述了如何使用激光捕获显微切割(LCM),以获得丰富的海马神经元群体或单个神经元,随后采用实时定量PCR和/或整个基因组微阵列基因表达分析的损伤大鼠的大脑冰冻切片。
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