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DOI :
10.3791/50317-v
May 7th, 2013
Chapters
0:05
Title
2:07
Preparation of Live Cell Imaging
4:06
ER-calcium Live Cell Imaging with an Inverted Laser Scanning Confocal Microscope
5:57
Image Processing and Data Analysis
6:38
Representative Endoplasmic Reticulum Calcium Imaging Results
8:18
Conclusion
2:50
Dye Loading of Cells
대상 - 에스테르 가수 분해 효소 유도 염료로드 (TED)는 형광 이미징에 의해 세포 내 칼슘 저장소 역학의 분석을 지원합니다. 방법은 합성 낮은 친화력 CA의 로컬 폭로을 향상 소포체 (ER)로 재조합 Carboxylesterase의 대상에 기지 2 +에 표시 염료.
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