Name: a simple method of mouse lung intubation
Uploaded: 2013-03-21T13:00:00
Description: Watch this Scientific Journal Video about A Simple Method of Mouse Lung Intubation at JoVE.com

支持由NIH HL-10342。

1。准备程序必须先取得并准备以下物品： <ol><li> 插管，气管插管20-35克小鼠，我们使用了1或1.5英寸长，20号静脉导管（BD Insylte，火花，MD或Jelco OPTIVA，卡尔斯巴德，加利福尼亚州）。一个新的无菌导管可以用于各小鼠，但也可以重复使用导管的灭菌后，通过在70％乙醇中浸泡过夜。虽然无论是咽也不是无菌的气管鼠标适当的清洁程序，包括使用无菌手套和工具?...

<ol>
	<li>Brown, R. H., Walters, D. M., Greenberg, R. S., Mitzner, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+method+of+endotracheal+intubation+and+pulmonary+functional+assessment+for+repeated+studies+in+mice.">A method of endotracheal intubation and pulmonary functional assessment for repeated studies in mice.</a> J. Appl. Physiol. 87, 2362-2365 (1999).</li><li>Walters, D. M., Wills-Karp, M., Mitzner, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Assessment+of+cellular+profile+and+lung+function+with+repeated+bronchoalveolar+lavage+in+individual+mice.">Assessment of cellular profile and lung function with repeated bronchoalveolar lavage in individual mice.</a> Physiol. Genomics. 2, 29-36 (2000).</li><li>Rivera, B., Miller, S., Brown, E., Price, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+novel+method+for+endotracheal+intubation+of+mice+and+rats+used+in+imaging+studies.">A novel method for endotracheal intubation of mice and rats used in imaging studies.</a> Contemporary Topics in Laboratory Animal Science / American Association for Laboratory Animal Science. 44, 52-55 (2005).</li><li>Hamacher, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microscopic+wire+guide-based+orotracheal+mouse+intubation:+description,+evaluation+and+comparison+with+transillumination.">Microscopic wire guide-based orotracheal mouse intubation: description, evaluation and comparison with transillumination.</a> Laboratory Animals. 42, 222-230 (2008).</li><li>Spoelstra, E. N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+novel+and+simple+method+for+endotracheal+intubation+of+mice.">A novel and simple method for endotracheal intubation of mice.</a> Laboratory Animals. 41, 128-135 (2007).</li><li>Zhao, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+technique+for+retrograde+intubation+in+mice.">A technique for retrograde intubation in mice.</a> Lab Animal. 35, 39-42 (2006).</li><li>Vergari, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+new+method+of+orotracheal+intubation+in+mice.">A new method of orotracheal intubation in mice.</a> European Review for Medical and Pharmacological Sciences. 8, 103-106 (2004).</li><li>Vergari, A., Polito, A., Musumeci, M., Palazzesi, S., Marano, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Video-assisted+orotracheal+intubation+in+mice.">Video-assisted orotracheal intubation in mice.</a> Laboratory Animals. 37, 204-206 (2003).</li><li>Hastings, R. H., Summers-Torres, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Direct+Laryngoscopy+in+Mice.">Direct Laryngoscopy in Mice.</a> Contemporary Topics in Laboratory Animal Science / American Association for Laboratory Animal Science. 38, 33-35 (1999).</li><li>MacDonald, K. D., Chang, H. Y., Mitzner, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+improved+simple+method+of+mouse+lung+intubation.">An improved simple method of mouse lung intubation.</a> J. Appl. Physiol. 106, 984-987 (2009).</li><li>Ewart, S. L., Levitt, R. C., Mitzner, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Respiratory+system+mechanics+in+mice+measured+by+end-inflation+occlusion.">Respiratory system mechanics in mice measured by end-inflation occlusion.</a> Journal of Applied Physiology. 79, 560-566 (1995).</li><li>MacDonald, K. D., McKenzie, K. R., Mitzner, W., Zeitlin, P. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Lung+Mechanics+in+Heterozygous+CF+Mice+after+Repeated+LPS+Dosing.">Lung Mechanics in Heterozygous CF Mice after Repeated LPS Dosing.</a> Am. J. Respir. Crit. Care Med. 175 (4), A930 (2007).</li></ol>

这里介绍的方法具有几个优点。首先，该装置是简单的和相对便宜的..制造该装置不需要任何特殊工具或昂贵的设备。用导管引入的光源也意味着，一个不能忽视的气管开口，介绍人靠近气管的开口。使用0.5毫米导引也供应，以尽量减少创伤，可能会出现的一个更大的套管的初始插入。这里我们注意到，类似的光学探针是从商业供应商（布伦特里科学，旅游Braintree，MA）。他们的移动设备使用一个...

布朗等人在1999年发表了一篇论文，描述小鼠肺1气管插管的方法。这种技术具有相当的实用，在做重复肺功能或支气管肺泡灌洗在纵向研究中的单个小鼠2。从那时起，原来的文件，已经有好几篇文章，描述了不同的方法对小鼠气管插管3-9。虽然所有这些方法，可以成功地使用，它们通常需要大量的训练或成本。如气管插管的主要问题之一是，接近气管待插入气管插管插管方法，插管本身阻止光的可视化，因此，它需要去的地方。因此，在最关键的时候插入变得盲目。在本文中，我们将展示如何简单，廉价消除这种可视化的问题，从而确保以相对较少的培训或气管插管成功经验。

<table border="1"><tbody><tr><td> 材料 </td><td> 公司 </td><td> 产品编号 </td><td> 评论 </td></tr><tr><td>气管插管套管</td><td> BD Insylte，火花，MD或Jelco OPTIVA，卡尔斯巴德，加利福尼亚州</td><td></td><td> 1英寸长，20号静脉注射（IV）导管</td></tr><tr><td>光纤电缆</td><td>爱特蒙特光学，巴林顿，新泽西州</td><td> NT02-542 </td><td>大约2英尺长，0.5毫米的光纤（通信级塑料光纤）。应圆润的边缘的纤维端插入气管的纤维≈2厘米的结束，然后让小圆圈拖动笔尖上的1000砂砾埃默里纸的几秒钟。 </td></tr><tr><td>光源</td><td>沃尔皮</td><td> NCL-150 </td><td>虽然我们使用了150-W卤素光源，任何同等光源，甚至以低得多的功率都可以使用。 </td></tr><tr><td>硅橡胶塞</td><td></td><td></td><td> A＃1硅橡胶塞适合本演示中使用的光源。不同的光源，可能需要不同的大小。 </td></tr><tr><td>小硅橡胶管</td><td>科尔 - 帕默</td><td> EW-96410-13 </td><td> A≈1.5厘米的硅橡胶管（内径0.8，4毫米外径） </td></tr><tr><td>直角支架</td><td></td><td></td><td>我们的有机玻璃，但constucted线或金属丝，以近乎垂直的角度，可以贴在按住鼠标可用于任何材料。 </td></tr></tbody></table>

a simple method of mouse lung intubation

插管小鼠肺功能测量一个简单的程序有几个优势，在纵向研究数量有限，昂贵的动物。没有这样做，这是更常规的原因之一是，它是比较困难的，尽管有几个已发表的研究描述的方法来实现它。在本文中，我们展示了一个过程，消除插管与此相关联的主要障碍之一，就是在整个可视化的气管插管时。该方法使用一个0.5毫米的光纤光源，作为介绍人直接的小鼠气管插管插入气管插管。我们表明，它是可以使用此过程测量在个别小鼠的肺力学过程中，至少几个星期的时间。这项技术可以被设置以相对较少的费用和专门知识，它可以常规完成以相对较少的培训。这应该麦E - 这是任何实验室定期进行气管插管，从而使小鼠个体的纵向研究中，通过使用每个鼠标作为自己的控制，从而最大限度地减少所需的老鼠的数量和提高统计能力。

作为评价的方法，我们使用了4个20周龄雄性BALB / c小鼠的平均体重（±SEM）27.7±0.40克的。他们连续五周进行了研究，其中的肺阻力的测定是使用如前面所述的一个系统11。每只小鼠用氯胺酮（100微克/克体重）和甲苯噻嗪（15微克/克体重）在盐水中通过IP注射麻醉。然后，他们被插管如上所述。如果有任何疑问，是否是在气管和食道插管，这可以使用一个小的牙科镜验证。保持镜像冷冻箱中?...

Watch this Scientific Journal Video about A Simple Method of Mouse Lung Intubation at JoVE.com

A Simple Method of Mouse Lung Intubation

本文介绍了一种striaghforward和有效的方法，插管小鼠肺功能测量或肺灌注，使小鼠恢复，并在以后的时间进行研究。该过程涉及一种廉价的光纤光源直接照射的气管。

一个简单的方法小鼠肺插管

A  simple procedure to intubate mice for pulmonary function measurements would  have several advantages in longitudinal studies with limited numbers or  ...

Research

JoVE Journal

Medicine

A Model of Disturbed Flow-Induced Atherosclerosis in Mouse Carotid Artery by Partial Ligation and a Simple Method of RNA Isolation from Carotid Endothelium

一个型号的不安流致动脉粥样硬化小鼠颈动脉部分结扎术和颈动脉内皮细胞的RNA分离的简单方法

Despite the well-known close association, direct evidence linking disturbed flow to atherogenesis has been lacking. We have recently used a modified ...

科研

医学

Guidelines for Elective Pediatric Fiberoptic Intubation

选修儿科光纤插管指南

Fiberoptic intubation in pediatric patients is often required especially in difficult airways of syndromic patients i.e. Pierre Robin Syndrome. Small ...

A Simple Guide Screw Method for Intracranial Xenograft Studies in Mice

一个简单的指南螺杆颅内移植瘤小鼠的研究方法

The grafting of human tumor cells into the brain of immunosuppressed mice is an established method for the study of brain cancers including glioblastoma ...

Noninvasive Intratracheal Intubation to Study the Pathology and Physiology of Mouse Lung

无创性气管内插管，以研究小鼠肺的病理生理

The use of a model that mimics the condition of lung diseases in humans is critical for studying the pathophysiology and/or etiology of a particular ...

Implantation of Fibrin Gel on Mouse Lung to Study Lung-specific Angiogenesis

纤维蛋白胶对小鼠肺移植研究肺特异性血管生成

Recent significant advances in stem cell research and bioengineering techniques have made great progress in utilizing biomaterials to regenerate and ...

Intubation-mediated Intratracheal (IMIT) Instillation: A Noninvasive, Lung-specific Delivery System

Intubation-mediated Intratracheal IMIT Instillation: A Noninvasive, Lung-specific Delivery System

插管介导气管内（IMIT）灌输：一种无创，龙专用输送系统

Respiratory disease studies typically involve the use of murine models as surrogate systems. However, there are significant physiologic differences ...

Mouse Pneumonectomy Model of Compensatory Lung Growth

补偿性增长龙鼠标肺切除型号

In humans, disrupted repair and remodeling of injured lung contributes to a host of acute and chronic lung disorders which may ultimately lead to ...

Method of Isolated Ex Vivo Lung Perfusion in a Rat Model: Lessons Learned from Developing a Rat EVLP Program

Method of Isolated Ex Vivo Lung Perfusion in a Rat Model: Lessons Learned from Developing a Rat EVLP Program

的隔离方法<em&gt;离体</em&gt;肺灌注的大鼠模型：教训制定鼠EVLP课程中学

The number of acceptable donor lungs available for lung transplantation is severely limited due to poor quality. Ex-Vivo Lung Perfusion (EVLP) has allowed ...

A Simple Device to Rapidly Prepare Whole Mounts of the Mouse Intestine

一个简单的设备，迅速准备小鼠肠的全坐骑

Preparing whole mounts of the mouse small intestine and colon for subsequent analysis or quantification can be time consuming and difficult. We describe ...

An Improved Method for Rapid Intubation of the Trachea in Mice

在小鼠气管插管快速的改进方法

Despite some anatomical and physiological differences, mouse models continue to be an essential tool for studying human lung disease. Bleomycin toxicity ...