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DOI :
10.3791/50354-v
September 10th, 2013
Chapters
0:05
Title
1:37
Assembly of Recombinant Core Histones into Octamers
5:32
Sedimentation Velocity Analysis of Reconstituted Nucleosomal Arrays
7:50
Results: Nucleosomal Arrays from Recombinant Xenopus Core Histones
9:56
Conclusion
3:14
Purification of Histone Octamers by Size Exclusion Chromatography |
的方法,提出了用于从重组核心组蛋白模型核小体阵列的重构和串联重复的核小体定位的DNA。我们还描述沉降速度实验在分析型超速离心机,和原子力显微镜(AFM)是如何被用来监测核小体阵列饱和的重构后的程度。
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