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DOI :
10.3791/50369-v
July 3rd, 2013
Chapters
0:05
Title
1:54
Preparation of Cells for siRNA and Reporter Transfection
5:42
Adding Transfection Mixtures to Cells
4:10
Preparing siRNA/DNA Transfection Mixtures
6:21
Determine Luciferase Activities from Cell Samples
8:04
Results: High-throughput Interrogation of Gene Function in Wnt/β-catenin, p53, and Kras Singaling
9:53
Conclusion
3:22
Preparing Reporter Construct DNA
3:19
siRNA and Reporter Transfection of Cells
細胞プロセスのシステム·レベルの理解を達成することは、現代の細胞生物学の目標です。我々はここでゲノム規模のRNAiライブラリーを用いた遺伝子の機能を調べるために、様々な細胞機能のエンドポイントのルシフェラーゼレポーターを多重化するための戦略について説明します。
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