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DOI :
10.3791/50386-v
June 24th, 2013
Chapters
0:05
Title
2:53
HeLa Cell Culture on Carbon-coated, Gold EM Finder Grids
3:54
HIV-1 Infection and Live-cell Imaging
5:19
Frozen-hydrated EM Sample Preparation
6:14
Cryo-fluorescence Light Microscopy
7:51
Cryo-electron Tomography
8:56
Three-dimensional Reconstruction using IMOD
9:33
Results: Observing Dynamic Behavior of Small HIV-1 Particles Interacting with Host HeLa Cells
12:45
Conclusion
우리는 고속 3D 라이브 세포 형광 현미경과 고해상도 cryo - 전자 단층 촬영을 결합하여 상호 현미경 방법을 설명합니다. 우리는 이미지 동적 호스트 헬라 세포와 상호 작용하는 작은 HIV-1 입자에 의해 상호 방식의 기능을 보여줍니다.
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