Name: accelerated type 1 diabetes induction in mice by adoptive transfer of diabetogenic cd4+ t cells
Uploaded: 2013-05-06T13:00:00
Description: Watch this Scientific Journal Video about Accelerated Type 1 Diabetes Induction in Mice by Adoptive Transfer of Diabetogenic CD4+ T Cells at JoVE.com

我们感谢博士。罗伯特BONNEAU和尼尔·克里斯滕森有益的意见。 这项工作是由美国宾夕法尼亚州立大学医学院的资金支持。

1。从脾和淋巴结肿大BDC2.5小鼠的T细胞的分离<ol><li>使用6周龄糖尿病前期致糖尿病的CD4 + T细胞的捐助者的的女BDC2.5小鼠。应该是无糖尿病老鼠尿葡萄糖测定（见下文）所确定的。 </li><li>安乐死每个鼠标采用CO 2窒息，在无菌条件下取出脾脏，腋窝和肱淋巴结。要取出脾脏，用70％乙醇浸泡皮草，再切和缩回皮肤。脾将是可见的，为暗红色的器官上的左侧的鼠标。 1英寸腹膜切口用小剪刀?...

<ol>
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D., Wang, B., Haskins, K., Benoist, C., Mathis, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Following+a+diabetogenic+T+cell+from+genesis+through+pathogenesis.">Following a diabetogenic T cell from genesis through pathogenesis.</a> Cell. 74, 1089-1100 (1993).</li><li>Stadinski, B. D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chromogranin+A+is+an+autoantigen+in+type+1+diabetes.">Chromogranin A is an autoantigen in type 1 diabetes.</a> Nat. Immunol. 11, 225-231 (2010).</li><li>Luhder, F., Chambers, C., Allison, J. P., Benoist, C., Mathis, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pinpointing+when+T+cell+costimulatory+receptor+CTLA-4+must+be+engaged+to+dampen+diabetogenic+T+cells.">Pinpointing when T cell costimulatory receptor CTLA-4 must be engaged to dampen diabetogenic T cells.</a> Proc. Natl. Acad. Sci. U.S.A. 97, 12204-12209 (2000).</li><li>Tang, Q., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vitro-expanded+antigen-specific+regulatory+T+cells+suppress+autoimmune+diabetes.">In vitro-expanded antigen-specific regulatory T cells suppress autoimmune diabetes.</a> J. Exp. Med. 199, 1455-1465 (2004).</li><li>Calderon, B., Suri, A., Pan, X. O., Mills, J. C., Unanue, E. 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1型糖尿病可以诱发受体小鼠的整个糖尿病NOD小鼠或来自致糖尿病性T细胞克隆的TCR转基因小鼠的脾细胞或T细胞亚群的过继转移在不同的效率。我们在此报告可重复的方法，在两周内100％的发病率在受体小鼠诱发T1D通过流式细胞仪纯化CD62L + BDC2.5转基因的CD4 + T细胞的转移到NOD.SCID小鼠。 这里所描述的特定优势的BDC2.5 T细胞传输模型，包括诱导时间很短，相比T1D月自发糖尿病和糖...

NOD小鼠自发地开发自身免疫性糖尿病，并已被广泛用作人I型糖尿病的动物模型1,2。 I型糖尿病NOD小鼠的发病机制中的浸润，其特征在于，开始在3-4周的年龄，胰腺胰岛的树突状细胞和巨噬细胞，T细胞和B细胞。这一阶段的非破坏性的周边胰岛炎导致一个缓慢的，逐渐产生胰岛素的胰腺β细胞的破坏，从而导致明显的糖尿病，由4-6个月3岁。已转让4,5脾细胞，CD4 + 6,7或CD8 + 8,9糖尿病NOD小鼠T细胞介导糖尿病NOD小鼠免疫功能低下，表明胰岛反应性T细胞在1型糖尿病的发病机制中发挥了核心作用。根据不同的实验条件下，糖尿病受体小鼠发展缓慢，在几个星期内，在这些研究中。同样，来自不同的T细胞克隆，费时和昂贵的致糖尿病性T细胞的培养，已报告的几个星期后，转移到受体小鼠7,10介导糖尿病。随着转基因小鼠表达的TCR来自CD4或CD8限制的致糖尿病的T细胞克隆，一些实验室的可用性，其后已表明，从小鼠脾脏T细胞能够转移糖尿病收件人11-13。具体来说，NOD小鼠BDC2.5只转基因BDC2.5 TCR，这是具体 ​​的嗜铬粒蛋白A，胰腺β细胞中的一种蛋白质14-16。 在体外激活或者未激活的全部或分馏公然糖尿病或糖尿病前期BDC2.5糖尿病小鼠转移到新生儿或免疫缺陷的NOD小鼠在不同的效率11,17-19脾细 ​​胞的转移。 我们描述了一个简单的方法，利用纯化的转基因的CD4 + T细胞在高效率和consiste的受体小鼠诱导T1D从糖尿病前期BDC2.5小鼠NCY。从这些小鼠中分离大量的幼稚，胰岛抗原特异性CD4 + T细胞通过荧光激活细胞分类（FACS），CD4 + CD62L + T细胞表达的转基因TCRVβ4的链。纯化的转基因T细胞，然后转移无需激活到NOD.SCID的老鼠，缺乏功能性T细胞和B细胞和胰岛炎和糖尿病无20。受体小鼠进行监测尿糖浓度升高，表明I型糖尿病，迅速发展后两周内的T细胞转移。 相比之下致糖尿病异构特异性T细胞转移的其他方法，我们的协议，使用流式细胞仪排序CD4 + T细胞，几乎完全表达的致糖尿病BDC2.5的TCR。由于他们的同质性，只有少数转移T细胞（约1×10 6个细胞/鼠标）T1D发展迅速，需要2个星期内，在100％的发病率。我们的协议的另一个优势是，irradiation的受体小鼠是没有必要的，因为它是为一些其他的方法。这种方法的一个潜在的局限性，它并没有允许调查，CD4和CD8 + T细胞亚群，或特别是CD8 + T细胞在糖尿病的贡献。 所描述的协议将是有用的研究的T1D的快速发展，介导的幼稚的，单特异性CD4 + T细胞，以及治疗策略干预胰岛抗原特异性的Th细胞的归巢至靶器官。

<table border="1" fo:keep-together.within-page="always">
	<tbody>
		<tr>
			<td>Name of Reagent/Material</td>
			<td>Company</td>
			<td>Catalog Number</td>
			<td>Comments</td>
		</tr>
		<tr>
			<td>BDC 2.5 TCR transgenic NOD mice (NOD.Cg-Tg(Tcr&alpha;BDC 2.5, Tcr&beta;BDC 2.5)</td>
			<td>JAX</td>
			<td>004460</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>NOD.SCID mice (NOD.CB17-Prkdcscid/J)</td>
			<td>JAX</td>
			<td>001303</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Dulbecco's Modified Eagle's Medium (DMEM)</td>
			<td>Themo Scientific</td>
			<td>SH30022.01</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Bayer Diastix</td>
			<td>Fisher Scientific</td>
			<td>AM2803</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>15 ml conical tubes</td>
			<td>Falcon</td>
			<td>352095</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>50 ml conical tubes</td>
			<td>Falcon</td>
			<td>352070</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Sterile surgical tweezers</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Sterile small pair scissors</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Sterile large pair scissors</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>70 &mu;m cell strainers</td>
			<td>Fisher Scientific</td>
			<td>22363548</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>35 &mu;m cell strainer cap tubes</td>
			<td>BD Biosciences</td>
			<td>352235</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Ammonium-Chloride-Potassium (ACK) buffer</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
			<td>0.15 M NH4Cl, 1 mM KHCO3, 0.1 mM Na2EDTA, pH 7.2 in dH2O</td>
		</tr>
		<tr>
			<td>BD FACSFlowTM sheath fluid</td>
			<td>BD Biosciences</td>
			<td>342003</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>FACS staining buffer</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
			<td>PBS, 0.2 mM EDTA, 0.5% BSA/FCS, filter sterilized</td>
		</tr>
		<tr>
			<td>Phase contrast microscope</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Trypan blue</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Hemocytometer</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Anti-CD4 (APC) mAb</td>
			<td>Biolegend</td>
			<td>1005616</td>
			<td>clone RM4-5</td>
		</tr>
		<tr>
			<td>Anti-TCR V&beta;4 (FITC) mAb</td>
			<td>BD Biosciences</td>
			<td>553365</td>
			<td>clone KT4</td>
		</tr>
		<tr>
			<td>Anti-CD62L (PE) mAb</td>
			<td>BD Biosciences</td>
			<td>553151</td>
			<td>clone MEL-14</td>
		</tr>
		<tr>
			<td>Cell sorter</td>
			<td>BD Biosciences</td>
			<td>&nbsp;</td>
			<td>e.g. BD FACSAria III</td>
		</tr>
		<tr>
			<td>Heat lamp</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Mouse restrainer</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>1 ml syringes</td>
			<td>Becton Dickinson</td>
			<td>309602</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>18-1&frac12; gauge needles (sterile)</td>
			<td>Becton Dickinson</td>
			<td>305196</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>27&frac12; gauge needles (sterile)</td>
			<td>Becton Dickinson</td>
			<td>305109</td>
			<td>&nbsp;</td>
		</tr>
	</tbody>
</table>

accelerated type 1 diabetes induction in mice by adoptive transfer of diabetogenic cd4+ t cells

非肥胖糖尿病（NOD）小鼠自发地开发自身免疫性糖尿病12周龄后，是最广泛研究的动物模型，人类1型糖尿病（1型糖尿病）。照射的受体小鼠的细胞转移的研究已经建立了1型糖尿病的发病机制在这个模型中，T细胞的关键。我们描述一个简单的方法迅速诱发T1D继转移纯化，小学的CD4 + T细胞前糖尿病NOD小鼠胰岛特定的T细胞受体（TCR）BDC2.5到NOD.SCID受体小鼠的转基因。这种技术的主要优点是可以在同一天，不要求收件人照射型糖尿病的发病率很高，引起T细胞转移后2周内完成隔离和致糖尿病性T细胞过继转移。因此，研究可以继续以更快的速度比I型糖尿病的发病机理和治疗干预的方法依赖于异质性T细胞群或克隆来自糖尿病NOD小鼠。

我们的研究结果表明隔离的转基因BDC2.5细胞表达CD62L，这是家二级淋巴器官，如胰腺淋巴结T细胞的关键。我们的研究结果进一步证明了强有力的能力，这个单特异性T细胞的人口转移迅速和有效地T1D到NOD.SCID受体小鼠。 图2中所示的分离的致糖尿病CD4 + T细胞源自BDC2.5老鼠的。每股供体小鼠细胞分选前获得约5×10 7细胞汇集脾和淋巴结肿大。流式细胞仪排序之...

Watch this Scientific Journal Video about Accelerated Type 1 Diabetes Induction in Mice by Adoptive Transfer of Diabetogenic CD4+ T Cells at JoVE.com

Accelerated Type 1 Diabetes Induction in Mice by Adoptive Transfer of Diabetogenic CD4+ T Cells

我们提供了一个可重复的方法，诱导1型糖尿病（T1D）在小鼠胰岛抗原特异性的，初级的CD4 + T细胞过继转移在两个星期内。

加速1型糖尿病感应在小鼠体内的过继转移致糖尿病的CD4 + T细胞

The nonobese diabetic (NOD) mouse spontaneously develops autoimmune diabetes after 12 weeks of age and is the most extensively studied animal model of ...

Research

JoVE Journal

Immunology and Infection

Experimental Metastasis and CTL Adoptive Transfer Immunotherapy Mouse Model

实验转移和CTL过继转移免疫小鼠模型

Experimental metastasis mouse model is a simple and yet physiologically relevant metastasis model. The tumor cells are injected intravenously (i.v) into ...

科研

免疫与感染

Preparation and Use of HIV-1 Infected Primary CD4+ T-Cells as Target Cells in Natural Killer Cell Cytotoxic Assays

制备和使用艾滋病毒感染主的CD4 + T细胞作为靶细胞，自然杀伤细胞的细胞毒性检测

 Natural killer (NK) cells are a vital component of the innate immune response to virus-infected cells. It is important to understand the ability of NK ...

Assessing the Innate Sensing of HIV-1 Infected CD4+ T Cells by Plasmacytoid Dendritic Cells Using an Ex vivo Co-culture System.

Assessing the Innate Sensing of HIV-1 Infected CD4+ T Cells by Plasmacytoid Dendritic Cells Using an Ex vivo Co-culture System.

评估的HIV-1感染的CD4的先天传感<sup&gt; +</sup&gt;性T细胞浆细胞样树突细胞使用<em&gt;体外</em&gt;共培养体系。

HIV-1 innate sensing requires direct contact of infected CD4+ T cells with plasmacytoid dendritic cells (pDCs). In order to study this process, the ...

Transplantation of Tail Skin to Study Allogeneic CD4 T Cell Responses in Mice

尾部皮肤来研究异基因CD4 + T细胞应答的小鼠移植

The study of T cell responses and their consequences during allo-antigen recognition requires a model that enables one to distinguish between donor and ...

Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice

Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice

诱导小鼠肠道炎症由效应CD4的过继转移<sup&gt; +</sup&gt; CD45RB<sup&gt;高</sup&gt; T细胞免疫缺陷小鼠

There are many different animal models available for studying the pathogenesis of human inflammatory bowel diseases (IBD), each with its own advantages ...

Preparation of CD4+ T Cells for Analysis of GD3 and GD2 Ganglioside Membrane Expression by Microscopy

Preparation of CD4+ T Cells for Analysis of GD3 and GD2 Ganglioside Membrane Expression by Microscopy

CD4的制备<sup&gt; +</sup&gt; T细胞通过显微镜分析GD3和GD2神经节苷脂膜表达的

The methods described herein for activation of na&#239;ve CD4+ T cells in suspension and their adherence in coverslips for confocal microscopy analysis ...

In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Na&#239;ve CD4+ T Cells Using a TGF-&#946;-containing Protocol

In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells iTregs from NaÃƒÆ’Ã†'Ãƒâ€šÃ‚Â¯ve CD4+ T Cells Using a TGF-ÃƒÆ’Ã…Â½Ãƒâ€šÃ‚Â²-containing Protocol

在从幼稚的CD4 + T细胞的人CD4 + FOXP3 +诱导调节性T细胞（iTregs的）使用含有TGF-β-协议的体外分化

Regulatory T cells (Tregs) are an integral part of peripheral tolerance, suppressing immune reactions against self-structures and thus preventing ...

Induction of Paralysis and Visual System Injury in Mice by T Cells Specific for Neuromyelitis Optica Autoantigen Aquaporin-4

诱导麻痹及视觉系统损伤小鼠 T 细胞特异的视神经脊髓炎抗原水通道蛋白-4

While it is recognized that aquaporin-4 (AQP4)-specific T cells and antibodies participate in the pathogenesis of neuromyelitis optica (NMO), a human ...

Stereotactic Adoptive Transfer of Cytotoxic Immune Cells in Murine Models of Orthotopic Human Glioblastoma Multiforme Xenografts

立体定向移植人胶质瘤多形性移植小鼠细胞毒性免疫细胞模型的研究

Glioblastoma multiforme (GBM), the most frequent and aggressive primary brain cancer in adults, is generally associated with a poor prognosis, and scarce ...