Name: assessment of cardiac function and myocardial morphology using small animal look-locker inversion recovery (salli) mri in rats
Uploaded: 2013-07-19T13:00:00
Description: Watch this Scientific Journal Video about Assessment of Cardiac Function and Myocardial Morphology Using Small Animal Look-locker Inversion Recovery SALLI MRI in Rats at JoVE.com

1。准备 1.1。麻醉的老鼠<ol><li>将大鼠预先填充到腔室与异氟醚（约3分钟，5％的腔室的大小而定）麻醉大鼠。 </li><li>一旦麻醉，取出大鼠和称重。 </li><li>将鼻子的大鼠维持麻醉的麻醉面罩（异氟醚2-3％氧气1升/分钟）。在异氟醚麻醉大鼠心脏率下降到300-340 BPM（BPM在小鼠380-420）。 </li></ol> 1.2。插入一个尾静脉插管<ol><li>洗净用温水（38℃）和肥皂大鼠的尾部...

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在这里，我们提出了一种方法来生成小动物多模态（电影MR反转恢复和T1地图）MR图像。小动物的研究中扮演着越来越重要的作用，在心血管疾病的研究和CMR是一个强大的工具，使我们能够学习的功能，结构和组织组成的心肌。然而，小动物CMR有许多独特的挑战，由于高心脏和心脏大小小。在这项技术的发展，改进的成像方法，包括更多的时间有效的方法将进一步发挥作用。 ?...

心肌炎的一个主要原因是急性心脏衰竭，猝死，慢性扩张性心肌病7。 CMR已经建立的测量功能和体内组织分析的金标准技术。新型成像技术和改进在成像不仅可以提高诊断心肌炎，而且还帮助的病理生理研究和治疗靶点的速度辨识8-10。小动物成像是研究心血管疾病的一个重要工具。常规使用的技术，如在临床CMR晚钆增强（LGE）不能很容易地转移到小动物CMR由于高动物的心脏速率，但已经可以显示11。小动物看储物柜反转恢复方法（SALLI）生成的多模态图像数据，全面评估心脏功能和形态（ 图1设置允许ND 3）。这里，我们表明，在详细地说，程序和设置用于小动物成像一个典型的SALLI协议。特别是，我们表明T1成像数据集的重建和分析。

<table border="1">
		<tbody>
		 <tr>
			<td>Name of Reagent/Material</td>
			<td>Company</td>
			<td>Catalog Number</td>
			<td>Comments</td>
		 </tr>
		 <tr>
			<td>Adhesive Tape (Silkafix)</td>
			<td>Lohmann und Rauscher</td>
			<td>34327</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Gadopentetat-Dimeglumin (Magnevist)</td>
			<td>Bayer</td>
			<td>G-00012163</td>
			<td>2mmol/Kg</td>
		 </tr>
		 <tr>
			<td>Introcan Safety-W (G24)</td>
			<td>B. Braun</td>
			<td>4254503-01</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Red Dot, Neonatal Monitoring Electrode with Pre-Attached Lead Wire</td>
			<td>3M</td>
			<td>2269T</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Skin glue (Histoacryl)</td>
			<td>B. Braun</td>
			<td>1050052</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Scales (Typ 440)</td>
			<td>Kern</td>
			<td>95088</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Skin desinfection (Softasept N)</td>
			<td>B. Braun Petzold</td>
			<td>360250</td>
			<td></td>
		 </tr>
		 <tr>
			<td>Thermometer </td>
			<td>LumaSense Technologies</td>
			<td>Luxtron 812</td>
			<td></td>
		 </tr>
		</tbody>
 </table>

assessment of cardiac function and myocardial morphology using small animal look-locker inversion recovery (salli) mri in rats

小动物磁共振成像研究心脏功能和心肌组织的变化是一个重要的工具。心脏速率高的小动物（200〜600次/分）先前限于CMR成像的作用。小动物查找储物柜的反转恢复（SALLI）是一个小动物来克服这个问题1 T1映射序列。 T1的地图提供组织改建和造影剂动力学的定量信息。另外，也可以检测弥漫性过程，如心肌间质纤维化或水肿1-6。此外，从一个单一的图像数据集，它能够检查心脏功能和心肌疤痕通过生成电影和反转恢复制备的晚期钆增强型MR图像1。 视频显示一步一步的程序进行小动物CMR成像。在这里，它是一个健康成年DAWL的带有安永大鼠，然而自然地，它可以扩展到不同心脏的小动物模型。

在这里，我们展示了从健康成年大鼠结果。精心准备的动物设置，我们之前描述的，它有可能获得一个稳定的心电图信号。类似人类心脏MRI协议，我们开始一项调查，以找出心。如果大鼠在线圈的位置是正确的，我们将继续与二腔观和四腔观检测为SALLI堆栈的几何形状。 的SALLI技术产生三种类型的在同一时间获得的图像。这是，首先，电影的图像，可以用于研究局部和全局的L...

Watch this Scientific Journal Video about Assessment of Cardiac Function and Myocardial Morphology Using Small Animal Look-locker Inversion Recovery SALLI MRI in Rats at JoVE.com

Assessment of Cardiac Function and Myocardial Morphology Using Small Animal Look-locker Inversion Recovery SALLI MRI in Rats

对比度增强心脏磁共振（CMR）成像允许全面<em&gt;在体内</em&gt;评估心血管疾病的小动物模型的心脏。下面我们就详细的程序执行CMR成像，重建和分析，用小动物锁储物柜反转恢复（SALLI）的大鼠。

使用小动物看更衣室反转恢复（SALLI）的MRI大鼠心脏功能和心肌形态学评估

Small animal magnetic resonance  imaging is an important tool to study cardiac function and changes in myocardial  tissue. The high heart rates of small ...

Research

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Medicine

Assessment of Cardiac Function and Myocardial Morphology Using Small Animal Look-locker Inversion Recovery (SALLI) MRI in Rats

Assessment of Cardiac Function and Energetics in Isolated Mouse Hearts Using 31P NMR Spectroscopy

Assessment of Cardiac Function and Energetics in Isolated Mouse Hearts Using 31P NMR Spectroscopy

在分离的小鼠使用红桃心功能和能量学的评估 31 P核磁共振

Bioengineered mouse models have become powerful research tools in determining causal relationships between molecular alterations and models of ...

科研

医学

Acute Myocardial Infarction in Rats

大鼠急性心肌梗死

With heart failure leading the cause of death in the USA (Hunt), biomedical research is fundamental to advance medical treatments for cardiovascular ...

MRI and PET in Mouse Models of Myocardial Infarction

MRI和PET心肌梗死小鼠模型

Myocardial infarction is one of the leading causes of death in the Western world. The similarity of the mouse heart to the human heart has made it an ...

Ascending Aortic Constriction in Rats for Creation of Pressure Overload Cardiac Hypertrophy Model

大鼠建立压力超负荷心肌肥厚模型在升主动脉缩窄

Ascending aortic constriction is the most common and successful surgical model for creating pressure overload induced cardiac hypertrophy and heart ...

Assessment of Right Ventricular Structure and Function in Mouse Model of Pulmonary Artery Constriction by Transthoracic Echocardiography

右心室结构和功能的肺动脉缩窄经胸超声小鼠模型的评估

Emerging clinical data support the notion that RV dysfunction is critical to the pathogenesis of cardiovascular disease and heart failure1-3. Moreover, ...

Echocardiographic and Histological Examination of Cardiac Morphology in the Mouse

超声心动图与小鼠心脏形态学的组织学检查

An increasing number of genetically modified mouse models has become available in recent years. Moreover, the number of pharmacological studies performed ...

In Vivo Evaluation of Fracture Callus Development During Bone Healing in Mice Using an MRI-compatible Osteosynthesis Device for the Mouse Femur

In Vivo Evaluation of Fracture Callus Development During Bone Healing in Mice Using an MRI-compatible Osteosynthesis Device for the Mouse Femur

在体内MRI 对小鼠股骨骨愈合过程中骨折愈伤组织发育的评价

Endochondral fracture healing is a complex process involving the development of fibrous, cartilaginous, and osseous tissue in the fracture callus. The ...

Normothermic Ex Situ Heart Perfusion in Working Mode: Assessment of Cardiac Function and Metabolism

工作模式下的心功能和代谢评估

The current standard method for organ preservation (cold storage, CS), exposes the heart to a period of cold ischemia that limits the safe preservation ...

Continuous Blood Sampling in Small Animal Positron Emission Tomography/Computed Tomography Enables the Measurement of the Arterial Input Function

小型动物正电子发射断层扫描/计算机断层扫描中的连续血液采样支持动脉输入函数的测量

For quantitative analysis and bio-kinetic modeling of positron emission tomography/computed tomography (PET/CT) data, the determination of the temporal ...

Echocardiographic Assessment of Cardiac Anatomy and Function in Adult Rats

成人大鼠心脏解剖与功能的超声心动图评估

The use of experimental animal models has become crucial in cardiovascular science. Most studies using rodent models are focused on two-dimensional ...