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17.5K Views
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14:06 min
April 16th, 2013
DOI :
10.3791/50402-v
Chapters
0:05
Title
2:12
Isolation of Mononuclear Cells from Umbilical Cord Blood
4:19
Modified B-cell Isolation from Mononuclear Cells Using MACS Separation
6:32
Antibody Labeling and Preparation for Cell Sorting
8:32
Cell Sorting Using the MoFlo XDP Flow Cytometer
11:07
Results: Isolation of Cord Blood Pre-cursor B-cell Subsets
13:13
Conclusion
Summary
ここでは、臍帯血から前駆体のサブセットB-細胞を単離するためのプロトコルを記述します。核酸の十分な量と質を細胞から抽出したDNAまたはRNAを利用して、その後のアッセイに使用することができる。
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