University of Missouri-Columbia

8 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Isolation of Precursor B-cell Subsets from Umbilical Cord Blood

Md Almamun¹, Jennifer L. Schnabel¹, Susan T. Gater², Jie Ning¹, Kristen H. Taylor¹

¹Department of Pathology and Anatomical Sciences, University of Missouri-Columbia, ²Laboratory for Infectious Disease Research, University of Missouri-Columbia

Here we describe a protocol for isolating subsets of precursor B-cells from umbilical cord blood. A sufficient quantity and quality of nucleic acids may be extracted from the cells and used in subsequent assays utilizing DNA or RNA.

Medicine

Photothrombosis-induced Focal Ischemia as a Model of Spinal Cord Injury in Mice

Hailong Li^*1, Gourav Roy Choudhury^*1, Nannan Zhang¹, Shinghua Ding¹

¹Department of Bioengineering, Dalton Cardiovascular Research Center, University of Missouri

Photothrombosis is a minimally invasive and highly reproducible procedure to induce focal ischemia in the spinal cord and serves as a model of spinal cord injury in mice.

Chemistry

Functionalization of Single-walled Carbon Nanotubes with Thermo-reversible Block Copolymers and Characterization by Small-angle Neutron Scattering

Youngkyu Han¹, Suk-kyun Ahn^2,3, Zhe Zhang^1,4, Gregory S. Smith¹, Changwoo Do¹

¹Biology and Soft Matter Division, Neutron Science Directorate, Oak Ridge National Laboratory, ²Center for Nanophase Materials Sciences, Oak Ridge National Laboratory, ³Department of Polymer Science and Engineering, Pusan National University, ⁴Jülich Center for Neutron Science, Forschungszentrum Jülich

A method for the functionalization of carbon nanotubes with structure-tunable polymeric encapsulation layers and structural characterization using small-angle neutron scattering is presented.

Behavior

Assessing Working Memory in Children: The Comprehensive Assessment Battery for Children – Working Memory (CABC-WM)

Kathryn Cabbage¹, Shara Brinkley², Shelley Gray², Mary Alt³, Nelson Cowan⁴, Samuel Green⁵, Trudy Kuo⁶, Tiffany P. Hogan¹

¹Communication Sciences and Disorders, MGH Institute of Health Professions, ²Speech and Hearing Science, Arizona State University, ³Speech, Language, and Hearing Sciences, University of Arizona, ⁴Department of Psychological Sciences, University of Missouri-Columbia, ⁵Sanford School of Social and Family Dynamics, Arizona State University, ⁶School of Social and Behavioral Sciences, New College of Interdisciplinary Arts and Sciences, Arizona State University - West

Working memory predicts a significant amount of variance for a variety of cognitive tasks, including speaking, reading, and writing. However, few tools are available to assess working memory in children. We present an innovative, computer-based battery that comprehensively assesses different components of working memory in school-age children.

Cancer Research

An Oncogenic Hepatocyte-Induced Orthotopic Mouse Model of Hepatocellular Cancer Arising in the Setting of Hepatic Inflammation and Fibrosis

Xiaoqiang Qi^*1,2,3, Emily Schepers^*1,2, Diego Avella^1,2, Eric T. Kimchi^1,2, Jussuf T. Kaifi^1,2, Kevin F. Staveley-O'Carroll^1,2, Guangfu Li^1,2,3

¹Department of Surgery, University of Missouri-Columbia, ²Ellis Fischel Cancer Center, University of Missouri-Columbia, ³Molecular Microbiology and Immunology, University of Missouri-Columbia

Here, we describe the development of a clinically relevant murine model of liver cancer recapitulating the typical immune features of hepatocellular cancer (HCC).

Neuroscience

Imaging Mitochondrial Ca²⁺ Uptake in Astrocytes and Neurons using Genetically Encoded Ca²⁺ Indicators (GECIs)

Nannan Zhang¹, Zhe Zhang^1,2, Ilker Ozden², Shinghua Ding^1,2

¹Dalton Cardiovascular Research Center, University of Missouri-Columbia, ²Department of Biomedical, Biological and Chemical Engineering, University of Missouri-Columbia

This proctocol aims to provide a method for in vitro and in vivo mitochondrial Ca²⁺ imaging in astrocytes and neurons.

Medicine

Robot-assisted Total Mesorectal Excision and Lateral Pelvic Lymph Node Dissection for Locally Advanced Middle-low Rectal Cancer

Chenhao Hu^*1,2,3, Zhe Zhang^*1,2,3, Lei Zhang^1,2,3, Ruihan Liu^2,3, Jun Yan^1,2,3, Qi Sun^1,2,3, Guanghui Wang¹, Junjun She^1,2,3

¹Department of General Surgery, The First Affiliated Hospital of Xian Jiaotong University, ²Center for Gut Microbiome Research, Med-X Institute, The First Affiliated Hospital of Xian Jiao tong University, ³Department of High Talent, The First Affiliated Hospital of Xian Jiaotong University

The robotic technique described herein aims to detail a stepwise approach to robot-assisted total mesorectal excision and lateral pelvic lymph node dissection for locally advanced (T3/T4) rectal cancer located below the peritoneal reflection.

Developmental Biology

Generation of a Mouse Artificial Decidualization Model with Ovariectomy for Endometrial Decidualization Research

Yang Zhang^*1,2,3, Zhe Zhang^*1,2,3, Nannan Kang^1,2,3, Xiaoqiang Sheng^1,2,3

¹Center for Reproductive Medicine and Obstetrics and Gynecology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, ²State Key Laboratory of Reproductive Medicine, Nanjing Medical University, ³Center for Molecular Reproductive Medicine, Nanjing University

Here, we describe the method of generating an artificial decidualization model using the ovariectomized mouse, a classic endometrial decidualization experiment in the research field of endometrial decidualization.