Name: construction of a preclinical multimodality phantom using tissue-mimicking materials for quality assurance in tumor size measurement
Uploaded: 2013-07-29T12:00:00
Description: Watch this Scientific Journal Video about Construction of a Preclinical Multimodality Phantom Using Tissue-mimicking Materials for Quality Assurance in Tumor Size Measurement at JoVE.com

Forfatterne er taknemmelige for Dr. Madsen ved University of Wisconsin-Madison og Cristel Baiu på Gammex Inc. for at rådgive om TM materialer. Forfatterne er også taknemmelig for, at Dr. Malcolm David Murray for at levere de metoder til at konstruere fantom.

1.. Phantom Design En tegning af prækliniske multimodale fantom er vist i figur 1 7, 8. Størrelsen af ​​fantomet er 38 mm i diameter og 115 mm i længden for at tillade fantom, der skal scannes i forskellige dyrearter scannere. Fantomet indeholder fem tumor-simulering test objekter (diameter: 14, 10, 7, 4 og 2 mm) placeret i en dybde på 10 mm inden for fantom. 2.. Silicone Mold Byggeri Silikone forme er ...

<ol>
	<li>Prasad, S. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=CT+tumor+measurement+for+therapeutic+response+assessment:+Comparison+of+unidimensional,+bidimensional,+and+volumetric+techniques-Initial+observations.">CT tumor measurement for therapeutic response assessment: Comparison of unidimensional, bidimensional, and volumetric techniques-Initial observations.</a> Radiology. 225 (2), 416-419 (2002).</li><li>Cortes, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparison+of+unidimensional+and+bidimensional+measurement+in+metastatic+non-small+cell+lung+cancer.">Comparison of unidimensional and bidimensional measurement in metastatic non-small cell lung cancer.</a> Br. J. Cancer. 87 (2), 158-160 (2002).</li><li>Saini, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Radiologic+measurement+of+tumor+size+in+clinical+trials:+past,+present,+and+future.">Radiologic measurement of tumor size in clinical trials: past, present, and future.</a> AJR Am. J. Roentgenol. 176 (2), 333-334 (2001).</li><li>Suzuki, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Radiologic+measurements+of+tumor+response+to+treatment:+practical+approaches+and+limitations.">Radiologic measurements of tumor response to treatment: practical approaches and limitations.</a> Radiographics. 28 (2), 329-344 (2008).</li><li>Therasse, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=RECIST+revisited:+A+review+of+validation+studies+on+tumour+assessment.">RECIST revisited: A review of validation studies on tumour assessment.</a> Eur. J. Cancer. 42 (8), 1031-1039 (2006).</li><li>O'Connor, P. B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quantitative+imaging+biomarkers+in+the+clinical+development+of+targeted+therapeutics:+current+and+future+perspectives.">Quantitative imaging biomarkers in the clinical development of targeted therapeutics: current and future perspectives.</a> Lancet Oncol. 9 (8), 766-776 (2008).</li><li>Lee, Y. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Preclinical+multimodality+phantom+design+for+quality+assurance+of+tumor+size+measurement.">Preclinical multimodality phantom design for quality assurance of tumor size measurement.</a> BMC Med. Phys. 11 (1), (2011).</li><li>Lee, Y. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=QA+procedures+for+multimodality+preclinical+tumor+drug+response+testing.">QA procedures for multimodality preclinical tumor drug response testing.</a> Med. Phys. 37 (9), 4806-4816 (2010).</li><li>Park, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Measuring+response+in+solid+tumors:+comparison+of+RECIST+and+WHO+response+criteria.">Measuring response in solid tumors: comparison of RECIST and WHO response criteria.</a> Jpn. J. Clin. Oncol. 33 (10), 533-537 (2003).</li><li>Eisenhauer, E. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=New+response+evaluation+criteria+in+solid+tumors:+revised+RECIST+guideline+(version+1.1).">New response evaluation criteria in solid tumors: revised RECIST guideline (version 1.1).</a> Eur. J. Cancer. 45 (2), 228-247 (2009).</li><li>D'Souza, W. D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tissue+mimicking+materials+for+a+multi-imaging+modality+prostate+phantom.">Tissue mimicking materials for a multi-imaging modality prostate phantom.</a> Med. Phys. 28 (4), 688-700 (2001).</li><li>Mitchell, M. D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Agarose+as+a+tissue+equivalent+phantom+material+for+NMR+images.">Agarose as a tissue equivalent phantom material for NMR images.</a> Magn. Reson. Imaging. 4 (3), 263-266 (1986).</li></ol>

Målet med denne artikel var at tilvejebringe metoder til at gøre TM materialer til multimodale billedbehandling og konstruere en præklinisk multimodalitet fantom som et QA værktøj til nøjagtig tumorstørrelse måling med forskellige modaliteter i flere institutioner. Som tidligere nævnt blev TM materialer oprindeligt blev udviklet af Dr. Ernest L. Madsen laboratorium ved University of Wisconsin Madison for en multi-afbildningsmodalitet prostata fantom. Vi ændrede Dr. Madsens TM materiale protokoller for vores eg...

<html> Vurdering af forandringen i tumorstørrelse er et vigtigt endepunkt til evaluering af aktiviteten af anti-tumor lægemidler i både tumor krympning og sygdomsprogression 9, 10. World Health Organization (WHO) og Response Evaluation Criteria i solide kræftsvulster (RECIST) er de kodificerede metoder til anatomiske vurdering af tumor læsioner i billeddiagnostiske metoder såsom ultralyd (US), computertomografi (CT) eller magnetisk resonans (MRI). For WHO kriterier er produktet af tumor maksimal diameter og dens største vinkelrette diameter i tværgående plan for målområder beregnede 4.. I modsætning til RECIST er summen af længste diametre i det tværgående plan for et begrænset antal target-læsioner beregnede 4.. På trods af konstant stigende interesse for tumor terapeutisk respons vurdering har der ikke været nogen prækliniske kvalitetssikring (QA) fantom / QA procedurer for imaging biomarkør. indhold &quot;&gt; I betragtning af at tumorstørrelse målinger baseret på WHO kriterier og / eller RECIST er den eneste imaging biomarkør er godkendt af den amerikanske Food and Drug Administration (FDA), som udgangspunkt for QA for eventuelle andre billeddiagnostiske biomarkører, Lee et al. konstrueret og fremstillet UTHSCSA / Gammex Mark 1 og Mark 2 fantomer til QA af tumorstørrelse måling i samarbejde med Gammex Inc 7.. Mark 1. phantom var en revideret udgave af en Gammex kommerciel amerikansk fantom og dermed størrelsen var for stor til at passe ind dyr CT og MR-skannere. Også nogle værktøjer i Mark 1 fantom var unødvendige for tumorstørrelse måling. Mark 2 phantom blev designet baseret på RECIST som er den seneste FDA-godkendt billeddannelse biomarkør. dog størrelsen af ​​Mark 2 fantom var stadig for stor til MR-scannere, og CT-og MR-billedkvaliteten af fantomet var ikke acceptabelt for nøjagtig tumorstørrelse måling 7.. QA phantom beskrev hanrein blev re-designet til at overvinde manglerne i de tidligere fantomer og konstrueret ved hjælp af modificerede tissue-efterlignende (TM) materialer og protokoller udviklet i vores laboratorium. Dette papir beskriver detaljerne i protokollerne for phantom konstruktion: Først metoder indføres for at forberede silikoneforme nødvendige for støbning tumor-simulering testgenstande og samle en rotator til at rotere et fantom at forhindre gravitation sedimentation. Sekund, protokoller for at forberede TM materialer modificeret fra D&#39;Souza et al. &#39;S for USA, CT og MR scanning er beskrevet 11. De fysiske egenskaber af TM materialer blev testet i hver modalitet at sikre, at TM materialer repræsenterede humane bløde væv som observeret i de billeder optaget med forskellige modaliteter, men resultaterne er ikke vist her. For det tredje er protokollen for fantom konstruktion beskrevet. Endelig er USA, CT og MR billeder af fantomet præsenteret som resultat.

<table border="1">
	<tbody>
		<tr>
			<td>Reagent/Material</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>PVC pipe</td>
			<td>N/A</td>
			<td>N/A</td>
			<td>Home Depot</td>
		</tr>
		<tr>
			<td>Bolt, nut, washer and metal plates</td>
			<td>N/A</td>
			<td>N/A</td>
			<td>Home Depot</td>
		</tr>
		<tr>
			<td>Acrylic plates and rods</td>
			<td>N/A</td>
			<td>N/A</td>
			<td>Plastic supply in San Antonio, TX</td>
		</tr>
		<tr>
			<td>Steel balls</td>
			<td>Nordex, Inc.</td>
			<td>AEC-M2-2, -4, -7, -10 and -14</td>
			<td>2, 4, 7, 10 and 14 mm diameter</td>
		</tr>
		<tr>
			<td>C-clamps</td>
			<td>Adjustable Clamp</td>
			<td>1420-C</td>
			<td>2 inch length</td>
		</tr>
		<tr>
			<td>Masking tape</td>
			<td>3M Industrial Adhesives and Tapes</td>
			<td>2600</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Duct tape</td>
			<td>3M Industrial Adhesives and Tapes</td>
			<td>S-3763SIL</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>J-B KWIK</td>
			<td>J-B WELD Co.</td>
			<td>380238</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>3M Scotch-Weld Epoxy Adhesive</td>
			<td>3M Industrial Adhesives and Tapes</td>
			<td>DP-100</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Silicone grease</td>
			<td>Permatex, Inc.</td>
			<td>22058</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Silicone glue</td>
			<td>DAP, Inc.</td>
			<td>688</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Silicone rubber compound</td>
			<td>Smooth-ON, Inc.</td>
			<td>Smooth-SilTM950 Part A and B</td>
			<td>A:B mix ratio = 10:1 by weight</td>
		</tr>
		<tr>
			<td>Brush</td>
			<td>N/A</td>
			<td>N/A</td>
			<td>Hobby Lobby</td>
		</tr>
		<tr>
			<td>Syringe</td>
			<td>Becton Dickinson</td>
			<td>309604</td>
			<td>10 ml</td>
		</tr>
		<tr>
			<td>Needle</td>
			<td>Becton Dickinson</td>
			<td>305156</td>
			<td>22-gauge 1.5 inch length</td>
		</tr>
		<tr>
			<td>Funnel</td>
			<td>N/A</td>
			<td>N/A</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Mesh filters</td>
			<td>Small parts, Inc.</td>
			<td>CMN-0010-C and CMN-0020-C</td>
			<td>10 and 20 &mu;m</td>
		</tr>
		<tr>
			<td>Whole milk</td>
			<td>N/A</td>
			<td>N/A</td>
			<td>HEB in San Antonio, TX</td>
		</tr>
		<tr>
			<td>Thimerosal</td>
			<td>Sigma-Aldrich Co.</td>
			<td>T5125</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Propanol</td>
			<td>Sigma-Aldrich Co.</td>
			<td>33538</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>EDTA</td>
			<td>Sigma-Aldrich Co.</td>
			<td>431788</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>CuCl2</td>
			<td>Sigma-Aldrich Co.</td>
			<td>459097</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Agarose</td>
			<td>Sigma-Aldrich Co.</td>
			<td>A0169</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>BaSO4</td>
			<td>Sigma-Aldrich Co.</td>
			<td>B8675</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Glass beads</td>
			<td>Potters Industries, Inc.</td>
			<td>3000E</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>PET/AL/LLDPE*</td>
			<td>Pechiney Plastic Packaging, Inc.</td>
			<td>Pechiney Spec 151</td>
			<td>Phantom cover material</td>
		</tr>
		<tr>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
			<td>*Polyethylene terephthalate/aluminum/linear low density polyethylene</td>
		</tr>
		<tr>
			<td>Equipment</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Rotisserie motor</td>
			<td>Brinkmann</td>
			<td>812-7103-S</td>
			<td>Home Depot</td>
		</tr>
		<tr>
			<td>Water bath 1</td>
			<td>Precision, Inc.</td>
			<td>Model: 282, Serial #: 601091552</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Water bath 2</td>
			<td>VWR, Inc.</td>
			<td>Model: 1212, Serial #: 08119606</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>Ultrasound</td>
			<td>Visualsonics</td>
			<td>Serial #: 770/120-259</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>CT</td>
			<td>Gamma Medica-Ideas</td>
			<td>Serial #: GR 0050</td>
			<td>&nbsp;</td>
		</tr>
		<tr>
			<td>MRI</td>
			<td>Bruker</td>
			<td>Part #: W3301390, Serial #: 0030</td>
			<td>&nbsp;</td>
		</tr>
	</tbody>
</table>

construction of a preclinical multimodality phantom using tissue-mimicking materials for quality assurance in tumor size measurement

World Health Organization (WHO), og Response Evaluation Criteria i solide tumorer (RECIST) arbejdsgrupper fortaler standardiserede kriterier for radiologisk vurdering af solide tumorer som reaktion på anti-tumor lægemiddelterapi i 1980&#39;erne og 1990&#39;erne, hhv. WHO kriterier måler solide tumorer i to dimensioner, mens RECIST målinger kun bruge én dimension, der anses for at være mere reproducerbar 1, 2, 3,4,5. Disse kriterier er ofte blevet brugt som den eneste imaging biomarkør er godkendt af den amerikanske Food and Drug Administration (FDA) 6.. For at måle tumor respons på anti-tumor stoffer på billeder med nøjagtighed, derfor er et robust kvalitetssikring (QA) procedurer og tilhørende QA fantom nødvendig. For at imødekomme dette behov, forfatterne konstrueret en præklinisk multimodalitet (for ultralyd (US), computertomografi (CT) og magnetisk resonans imaging (MRI)) fantom hjælp tissue-efterlignende (TM)materialer baseret på det begrænsede antal target-læsioner kræves af RECIST ved at revidere en Gammex amerikanske kommercielle phantom 7.. Tillægget til Lee et al. Viser procedurerne i phantom fabrikation 7.. I denne artikel er alle protokoller indført i en trin-for-trin mode begynder med procedurer for udarbejdelsen af ​​silikone forme til støbning tumor-simulering test objekter i fantom, efterfulgt af tilberedning af TM materialer til multimodalitet billeddannelse, og endelig opførelsen af præklinisk multimodalitet QA fantom. Det primære formål med dette oplæg er at give de protokoller til at tillade alle interesserede i selvstændigt konstruere et fantom til deres egne projekter. QA procedurer for tumorstørrelse måling, og RECIST, WHO og volumen måleresultater af test objekter lavet på flere institutioner, der anvender denne QA fantom er vist i detaljer i Lee et al. 8..

Figur 3 B og Figur 5 viser to silikone forme til støbning test objekter, og multimodalitet fantom hhv. Den længde × bredde × dybde hver form er 109 mm × 37 mm × 21 mm og to forme er identiske spejlbilleder. En støbeform har 1 mm huller hvor TM materiale kan indsættes ved hjælp af en tynd nål. Hver form har yderligere fem huller til justering stænger. Den længde × bredde × dybde af fantomet er 115 mm × 38 mm × 24 mm, og dens oprindelige masse var 101,02 gram. Størrelsen ...

Watch this Scientific Journal Video about Construction of a Preclinical Multimodality Phantom Using Tissue-mimicking Materials for Quality Assurance in Tumor Size Measurement at JoVE.com

Construction of a Preclinical Multimodality Phantom Using Tissue-mimicking Materials for Quality Assurance in Tumor Size Measurement

Dette papir beskriver interne procedurer at konstruere en præklinisk multimodalitet fantom lavet af væv-efterlignende (TM) materialer til kvalitetssikring (QA) i tumorstørrelse måling i animalske billeddiagnostiske metoder såsom ultralyd (US), computertomografi (CT) og magnetisk resonans (MRI).

Opførelse af en præklinisk Multimodality Phantom Brug Tissue-efterlignende Materialer kvalitetssikring i tumorstørrelsen Measurement

World Health Organization (WHO) and the Response Evaluation Criteria in Solid Tumors (RECIST) working groups advocated standardized criteria for ...

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