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DOI :
10.3791/50433-v
May 20th, 2013
Chapters
0:05
Title
1:20
Sample Extraction from Cells
2:34
Re-suspension and Filtration of Samples for UPLC
3:49
UPLC and Mass Spectrometer Setup
6:37
Differential Analysis
8:47
Results: Analysis of Metabolites Using UPLC-HRMS
10:42
Conclusion
不相关的代谢组学研究提供了一个假说,产生一种代谢轮廓的快照。此协议将展示从细胞,血清或组织中的代谢产物的提取和分析。代谢物甲范围进行调查,采用液 - 液固相萃取,微流超高效液相色谱/高分辨率质谱(UPLC-HRMS)耦合到差分分析软件。
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