Name: a laser-induced mouse model of chronic ocular hypertension to characterize visual defects
Uploaded: 2013-08-14T14:00:00
Description: Watch this Scientific Journal Video about A Laser-induced Mouse Model of Chronic Ocular Hypertension to Characterize Visual Defects at JoVE.com

本文所载的工作已经得到了青光眼研究由美国健康援助基金会（XL），威廉＆玛丽格雷夫特别学术研究奖，以防止失明（XL），博士道格拉斯·约翰逊奖伊利诺伊防盲协会（HC）和美国国立卫生研究院：授予R01EY019034（XL）。

程序 C57BL/6J小鼠（杰克逊实验室的Bar Harbor，ME）提出在西北大学的动物护理设施。按照协议，由西北大学机构动物护理和使用委员会，符合使用动物在神经科学的研究由美国国立卫生研究院的指引批准用于所有动物。 1。激光光凝从先前公布的5-7协议的程序修改光凝。 <ol><li>麻醉40-60日龄鼠标，腹腔内注射氯胺酮（100毫克/公斤...

<ol>
	<li>Gupta, N., Yucel, Y. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Glaucoma+as+a+neurodegenerative+disease.">Glaucoma as a neurodegenerative disease.</a> Curr. Opin. Ophthalmol. 18, 110-114 (2007).</li><li>Quigley, H. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neuronal+death+in+glaucoma.">Neuronal death in glaucoma.</a> Prog. Retin. Eye Res. 18, 39-57 (1999).</li><li>McKinnon, S. J., Schlamp, C. L., Nickells, R. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mouse+models+of+retinal+ganglion+cell+death+and+glaucoma.">Mouse models of retinal ganglion cell death and glaucoma.</a> Experimental Eye Research. 88, 816-824 (2009).</li><li>Pang, I. H., Clark, A. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rodent+models+for+glaucoma+retinopathy+and+optic+neuropathy.">Rodent models for glaucoma retinopathy and optic neuropathy.</a> J. Glaucoma. 16, 483-505 (2007).</li><li>Levkovitch-Verbin, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Translimbal+laser+photocoagulation+to+the+trabecular+meshwork+as+a+model+of+glaucoma+in+rats.">Translimbal laser photocoagulation to the trabecular meshwork as a model of glaucoma in rats.</a> Investigative Ophthalmology &amp; Visual Science. 43, 402-410 (2002).</li><li>Aihara, M., Lindsey, J. D., Weinreb, R. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Experimental+mouse+ocular+hypertension:+establishment+of+the+model.">Experimental mouse ocular hypertension: establishment of the model.</a> Investigative Ophthalmology &amp; Visual Science. 44, 4314-4320 (2003).</li><li>Grozdanic, S. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Laser-induced+mouse+model+of+chronic+ocular+hypertension.">Laser-induced mouse model of chronic ocular hypertension.</a> Investigative ophthalmology &amp; visual science. 44, 4337-4346 (2003).</li><li>Sappington, R. M., Carlson, B. J., Crish, S. D., Calkins, D. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+microbead+occlusion+model:+a+paradigm+for+induced+ocular+hypertension+in+rats+and+mice.">The microbead occlusion model: a paradigm for induced ocular hypertension in rats and mice.</a> Investigative ophthalmology &amp; visual science. 51, 207-216 (2010).</li><li>Ding, C., Wang, P., Tian, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+general+anesthetics+on+IOP+in+elevated+IOP+mouse+model.">Effect of general anesthetics on IOP in elevated IOP mouse model.</a> Experimental Eye Research. 92, 512-520 (2011).</li><li>Kalesnykas, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Retinal+ganglion+cell+morphology+after+optic+nerve+crush+and+experimental+glaucoma.">Retinal ganglion cell morphology after optic nerve crush and experimental glaucoma.</a> Investigative Ophthalmology &amp; Visual Science. 53, 3847-3857 (2012).</li><li>Shareef, S. R., Garcia-Valenzuela, E., Salierno, A., Walsh, J., Sharma, S. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chronic+ocular+hypertension+following+episcleral+venous+occlusion+in+rats.">Chronic ocular hypertension following episcleral venous occlusion in rats.</a> Experimental Eye Research. 61, 379-382 (1995).</li><li>Chiu, K., Chang, R., So, K. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Laser-induced+chronic+ocular+hypertension+model+on+SD+rats.">Laser-induced chronic ocular hypertension model on SD rats.</a> J. Vis. Exp. (10), e549 (2007).</li><li>Fu, C. T., Sretavan, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Laser-induced+ocular+hypertension+in+albino+CD-1+mice.">Laser-induced ocular hypertension in albino CD-1 mice.</a> Investigative Ophthalmology &amp; Visual Science. 51, 980-990 (2010).</li><li>Rangarajan, K. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Detection+of+visual+deficits+in+aging+DBA/2J+mice+by+two+behavioral+assays.">Detection of visual deficits in aging DBA/2J mice by two behavioral assays.</a> Curr. Eye Res. 36, 481-491 (2011).</li><li>Wang, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Direction-specific+disruption+of+subcortical+visual+behavior+and+receptive+fields+in+mice+lacking+the+beta2+subunit+of+nicotinic+acetylcholine+receptor.">Direction-specific disruption of subcortical visual behavior and receptive fields in mice lacking the beta2 subunit of nicotinic acetylcholine receptor.</a> J. Neurosci. 29, 12909-12918 (2009).</li><li>Douglas, R. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Independent+visual+threshold+measurements+in+the+two+eyes+of+freely+moving+rats+and+mice+using+a+virtual-reality+optokinetic+system.">Independent visual threshold measurements in the two eyes of freely moving rats and mice using a virtual-reality optokinetic system.</a> Visual Neuroscience. 22, 677-684 (2005).</li><li>Prusky, G. T., Alam, N. M., Beekman, S., Douglas, R. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rapid+quantification+of+adult+and+developing+mouse+spatial+vision+using+a+virtual+optomotor+system.">Rapid quantification of adult and developing mouse spatial vision using a virtual optomotor system.</a> Investigative Ophthalmology &amp; Visual Science. 45, 4611-4616 (2004).</li><li>Morrison, J. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+rat+model+of+chronic+pressure-induced+optic+nerve+damage.">A rat model of chronic pressure-induced optic nerve damage.</a> Experimental Eye Research. 64, 85-96 (1997).</li><li>Cone, F. E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+effects+of+anesthesia,+mouse+strain+and+age+on+intraocular+pressure+and+an+improved+murine+model+of+experimental+glaucoma.">The effects of anesthesia, mouse strain and age on intraocular pressure and an improved murine model of experimental glaucoma.</a> Experimental Eye Research. 99, 27-35 (2012).</li><li>Liu, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Brain-derived+neurotrophic+factor+and+TrkB+modulate+visual+experience-dependent+refinement+of+neuronal+pathways+in+retina.">Brain-derived neurotrophic factor and TrkB modulate visual experience-dependent refinement of neuronal pathways in retina.</a> J. Neurosci. 27, 7256-7267 (2007).</li><li>Liu, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Regulation+of+neonatal+development+of+retinal+ganglion+cell+dendrites+by+neurotrophin-3+overexpression.">Regulation of neonatal development of retinal ganglion cell dendrites by neurotrophin-3 overexpression.</a> The Journal of Comparative Neurology. 514, 449-458 (2009).</li><li>Sun, W., Li, N., He, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Large-scale+morphological+survey+of+mouse+retinal+ganglion+cells.">Large-scale morphological survey of mouse retinal ganglion cells.</a> The Journal of Comparative Neurology. 451, 115-126 (2002).</li><li>Feng, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sustained+Ocular+Hypertension+Induces+Dendritic+Degeneration+of+Mouse+Retinal+Ganglion+Cells+that+Depends+on+Cell-type+and+Location.">Sustained Ocular Hypertension Induces Dendritic Degeneration of Mouse Retinal Ganglion Cells that Depends on Cell-type and Location.</a> Investigative Ophthalmology &amp; Visual Science. , (2013).</li></ol>

我们报告以上，持续的高眼压可诱发小鼠眼睛的激光照射。盐水注射模型18和静脉烧灼模型的11两者都需要广泛的显微技能相比，激光照射是相对简单和容易执行的。通常情况下，我们就可以进行激光照射4-6小鼠在2-3小时。实现持续的高眼压的关键步骤是前房压扁之前，激光和激光照射的参数。排水便于目标激光的小梁区和减少伤害到附近的睫状体和血管6的前房中的流体?...

<table border="1">
 <tbody>
 <tr>
 <td></td>
 <td></td>
 <td></td>
 <td>Reagent</td>
 </tr>
 <tr>
 <td>moxifloxacin</td>
 <td>Alcon Labs, Inc.</td>
 <td>NDC 0065-4013-03</td>
 <td>0.5 %, Rx only</td>
 </tr>
 <tr>
 <td>Proparacaine Hydrochloride</td>
 <td>Bausch &amp; Lomb</td>
 <td>NDC 24208-730-06</td>
 <td>0.5 %, Rx only</td>
 </tr>
 <tr>
 <td>Ophthalmic Solution USP</td>
 <td>Bausch &amp; Lomb</td>
 <td>NDC 24208-730-06</td>
 <td>.5 %, Rx only</td>
 </tr>
 <tr>
 <td>ketamine</td>
 <td>Butler Schein Animal Health</td>
 <td>NDC 11695-0550-1</td>
 <td>100 mg / kg </td>
 </tr>
 <tr>
 <td>xylazine</td>
 <td>LLOYD Inc. of Iowa</td>
 <td>NADA 139-236</td>
 <td>10 mg / kg </td>
 </tr>
 <tr>
 <td>atropine sulfate solution</td>
 <td>Alcon Labs, Inc.</td>
 <td>NDC 61314-303-02</td>
 <td>1 %, Rx only</td>
 </tr>
 <tr>
 <td></td>
 <td></td>
 <td></td>
 <td>Equipment </td>
 </tr>
 <tr>
 <td>Slit Lamp, TOPCON </td>
 <td>Visual Systems Inc</td>
 <td>SL-3E</td>
 <td>powered by PS-30A</td>
 </tr>
 <tr>
 <td>OptoMotry 1.8.0 virtual</td>
 <td>CerebralMechanics Inc.</td>
 <td></td>
 <td></td>
 </tr>
 <tr>
 <td>opto-kinetic testing system</td>
 <td>CerebralMechanics Inc.</td>
 <td></td>
 <td></td>
 </tr>
 <tr>
 <td>Tonometer, TonoLab, for mice</td>
 <td>Colonial Medical Supply</td>
 <td></td>
 <td></td>
 </tr>
 <tr>
 <td>Heating pad</td>
 <td>Sunbeam Products Inc</td>
 <td>722-810</td>
 <td></td>
 </tr>
 <tr>
 <td>Argon laser </td>
 <td>Coherent Inc</td>
 <td>Ultima 2000SE</td>
 <td></td>
 </tr>
 <tr>
 <td>DECAPICONE Plastic cone holder </td>
 <td>Braintree Sci Inc.</td>
 <td>MDC-200</td>
 <td>for mouse</td>
 </tr>
 </tbody>
 </table>

a laser-induced mouse model of chronic ocular hypertension to characterize visual defects

经常伴有高眼压（IOP），青光眼，失明的主要原因之一。我们试图建立高眼压症的小鼠模型来模拟人类高眼压性青光眼。这里激光照射施加到角膜缘photocoagulate水外流，诱导闭角。使用激光治疗之前和之后的反弹式眼压计的眼压的变化进行监测。是用于测量相应的变化在视觉能力一个optomotor的行为测试。代表性的结果显示从一个鼠标开发激光照射后持续高眼压。下降的视力，对比敏感度观察在高眼压鼠标。总之，我们的研究引入了一个有价值的模型系统研究青光眼的小鼠神经元变性的分子机制。

中所描述的程序，目的是激光照明在角膜缘的区域photocoagulate水外流的小梁，诱导闭角型青光眼（ 图1）。大多数光刻眼睛表现出没有明显的物理伤害，色素脱落或感染，与以前的研究结果相一致6。当一小群​​小鼠（小于5％所有的光刻动物）展出，如的瘪眼球，严重的白内障，显着的色素支队，或出血严重损害的体征，我们立刻处死他们。约有30％的光刻眼睛轻微的角膜疤?...

Watch this Scientific Journal Video about A Laser-induced Mouse Model of Chronic Ocular Hypertension to Characterize Visual Defects at JoVE.com

A Laser-induced Mouse Model of Chronic Ocular Hypertension to Characterize Visual Defects

慢性高眼压诱导使用激光光凝小梁在小鼠眼睛。激光治疗后的几个月内升高的眼压（IOP）。减少实验动物的视力和对比敏感度使用optomotor测试监测。

激光诱导的慢性高眼压表征视觉缺陷的小鼠模型

Glaucoma, frequently associated with elevated intraocular pressure (IOP), is one of the leading causes of blindness. We sought to establish a mouse model ...

Research

JoVE Journal

Medicine

Driving Simulation in the Clinic: Testing Visual Exploratory Behavior in Daily Life Activities in Patients with Visual Field Defects

驾驶模拟诊所：测试视觉与视野缺损的患者在日常活动中的探索行为

Patients suffering from homonymous hemianopia after infarction of the posterior cerebral artery (PCA) report different degrees of constraint in daily ...

科研

医学

5/6th Nephrectomy in Combination with High Salt Diet and Nitric Oxide Synthase Inhibition to Induce Chronic Kidney Disease in the Lewis Rat

5/6th联合切除术，高盐饮食和一氧化氮合酶抑制剂诱导Lewis大鼠慢性肾脏病

Chronic kidney disease (CKD) is a global problem. Slowing CKD progression is a major health priority. Since CKD is characterized by complex derangements ...

Adult Mouse Venous Hypertension Model: Common Carotid Artery to External Jugular Vein Anastomosis.

成年小鼠静脉高压型号：颈总动脉，以颈外静脉吻合。

The understanding of the pathophysiology of brain arteriovenous malformations and arteriovenous fistulas has improved thanks to animal models. A rat model ...

Assessment of Vascular Function in Patients With Chronic Kidney Disease

血管功能在慢性肾脏疾病的评估

Patients with chronic kidney disease (CKD) have significantly increased risk of cardiovascular disease (CVD) compared to the general population, and this ...

A Mouse Model for Laser-induced Choroidal Neovascularization

小鼠模型的激光诱导脉络膜新生血管

The mouse laser-induced choroidal neovascularization (CNV) model has been a crucial mainstay model for neovascular age-related macular degeneration (AMD) ...

A Magnetic Microbead Occlusion Model to Induce Ocular Hypertension-Dependent Glaucoma in Mice

A磁条微珠缺血模型，以诱导小鼠高眼压，青光眼相关

The use of rodent models of glaucoma has been essential to understand the molecular mechanisms that underlie the pathophysiology of this multifactorial ...

A Simple Mechanical Procedure to Create Limbal Stem Cell Deficiency in Mouse

一个简单的机械过程来创建在小鼠角膜缘干细胞缺乏症

Limbal stem cell deficiency (LSCD) is a state of malfunction or loss of limbal epithelial stem cells, after which the corneal epithelium is replaced with ...

Induction and Phenotyping of Acute Right Heart Failure in a Large Animal Model of Chronic Thromboembolic Pulmonary Hypertension

慢性血栓栓塞性肺动脉高压大型动物模型中急性右心衰竭的诱导和表型分析

The development of acute right heart failure (ARHF) in the context of chronic pulmonary hypertension (PH) is associated with poor short-term outcomes. The ...

Isometric Contractility Measurement of the Mouse Mesenteric Artery Using Wire Myography

用线 Myography 测定小鼠肠系膜动脉的等轴收缩力

The wire myograph technique is used to assess the contractility of vascular smooth muscles in response to depolarization, GPCR agonists/inhibitors and ...

A Reversible Silicon Oil-Induced Ocular Hypertension Model in Mice

小鼠中可逆硅油诱发眼部高血压模型

Elevated intraocular pressure (IOP) is a well-documented risk factor for glaucoma. Here we describe a novel, effective method for consistently inducing ...