Name: a laser-induced mouse model of chronic ocular hypertension to characterize visual defects
Uploaded: 2013-08-14T14:00:00
Description: Watch this Scientific Journal Video about A Laser-induced Mouse Model of Chronic Ocular Hypertension to Characterize Visual Defects at JoVE.com

De werkzaamheden die in dit document is ondersteund door de Dr Douglas H. Johnson Award voor Glaucoom Research van de American Health Assistance Foundation (XL), de William &amp; Mary Greve Special Scholar Award van het Onderzoek ter voorkoming van Blindheid (XL), de Illinois Vereniging ter Voorkoming van Blindheid (HC) en NIH-subsidie ​​R01EY019034 (XL).

Procedures C57BL/6J-muizen (Jackson Laboratory, Bar Harbor, ME) worden opgewekt aan de Northwestern University&#39;s Animal Care Facility. Alle dieren worden gebruikt in overeenstemming met de door de Northwestern University Institutional Animal Care en gebruik Comite en gelijkvormig aan de richtlijnen inzake het gebruik van dieren in Neuroscience Research van de NIH goedgekeurde protocollen. 1. Laserfotocoagulatie De procedure van laserfo...

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Wij rapporteren boven die aanhoudende oculaire hypertensie kan worden geïnduceerd door laser verlichting in de muis ogen. Vergeleken met de zoutoplossing model 18 en de ader cautery model 11 beide vereisen uitgebreide microchirurgische vaardigheden, de laser verlichting is relatief eenvoudig en gemakkelijk uit te voeren. Meestal kunnen we de laser verlichting voeren voor 4-6 muizen in 2-3 uur. De kritische stappen naar duurzame IOP hoogte te bereiken, zijn de voorste kamer afvlakken voordat laser ...

<table border="1">
 <tbody>
 <tr>
 <td></td>
 <td></td>
 <td></td>
 <td>Reagent</td>
 </tr>
 <tr>
 <td>moxifloxacin</td>
 <td>Alcon Labs, Inc.</td>
 <td>NDC 0065-4013-03</td>
 <td>0.5 %, Rx only</td>
 </tr>
 <tr>
 <td>Proparacaine Hydrochloride</td>
 <td>Bausch &amp; Lomb</td>
 <td>NDC 24208-730-06</td>
 <td>0.5 %, Rx only</td>
 </tr>
 <tr>
 <td>Ophthalmic Solution USP</td>
 <td>Bausch &amp; Lomb</td>
 <td>NDC 24208-730-06</td>
 <td>.5 %, Rx only</td>
 </tr>
 <tr>
 <td>ketamine</td>
 <td>Butler Schein Animal Health</td>
 <td>NDC 11695-0550-1</td>
 <td>100 mg / kg </td>
 </tr>
 <tr>
 <td>xylazine</td>
 <td>LLOYD Inc. of Iowa</td>
 <td>NADA 139-236</td>
 <td>10 mg / kg </td>
 </tr>
 <tr>
 <td>atropine sulfate solution</td>
 <td>Alcon Labs, Inc.</td>
 <td>NDC 61314-303-02</td>
 <td>1 %, Rx only</td>
 </tr>
 <tr>
 <td></td>
 <td></td>
 <td></td>
 <td>Equipment </td>
 </tr>
 <tr>
 <td>Slit Lamp, TOPCON </td>
 <td>Visual Systems Inc</td>
 <td>SL-3E</td>
 <td>powered by PS-30A</td>
 </tr>
 <tr>
 <td>OptoMotry 1.8.0 virtual</td>
 <td>CerebralMechanics Inc.</td>
 <td></td>
 <td></td>
 </tr>
 <tr>
 <td>opto-kinetic testing system</td>
 <td>CerebralMechanics Inc.</td>
 <td></td>
 <td></td>
 </tr>
 <tr>
 <td>Tonometer, TonoLab, for mice</td>
 <td>Colonial Medical Supply</td>
 <td></td>
 <td></td>
 </tr>
 <tr>
 <td>Heating pad</td>
 <td>Sunbeam Products Inc</td>
 <td>722-810</td>
 <td></td>
 </tr>
 <tr>
 <td>Argon laser </td>
 <td>Coherent Inc</td>
 <td>Ultima 2000SE</td>
 <td></td>
 </tr>
 <tr>
 <td>DECAPICONE Plastic cone holder </td>
 <td>Braintree Sci Inc.</td>
 <td>MDC-200</td>
 <td>for mouse</td>
 </tr>
 </tbody>
 </table>

a laser-induced mouse model of chronic ocular hypertension to characterize visual defects

Glaucoom, vaak geassocieerd met verhoogde intraoculaire druk (IOP), een van de belangrijkste oorzaken van blindheid. We wilden een muismodel van oculaire hypertensie te bootsen menselijke high-drukglaucoom stellen. Hier laser verlichting wordt toegepast op het corneale limbus de waterige uitstroom photocoagulate, induceren geslotenhoekglaucoom. De veranderingen van IOP worden geanalyseerd met een rebound tonometer voor en na de laserbehandeling. Een optomotor gedragstest wordt gebruikt om overeenkomstige wijzigingen in de visuele capaciteit te meten. De vertegenwoordiger resultaat van de ene muis die opgelopen IOP hoogte ontwikkeld na de laser verlichting wordt getoond. Een verminderde gezichtsscherpte en contrastgevoeligheid wordt waargenomen in deze oculaire hypertensie muis. Samen, onze studie introduceert een waardevol modelsysteem om neuronale degeneratie en de onderliggende moleculaire mechanismen te onderzoeken in glaucomateuze muizen.

Zoals beschreven in de procedures wordt laserbelichting gericht op het trabeculaire maaswerk van het limbale gebied van de waterige uitstroom photocoagulate, induceren geslotenhoekglaucoom (figuur 1). De meeste gelaserde ogen vertoonden geen significante fysieke schade, pigment onthechting of infectie, in overeenstemming met eerdere bevindingen 6. Bij een kleine groep muizen (minder dan 5% van alle gelaserde dieren) vertoonden lichamelijke tekenen van ernstige schade, zoals leeggelopen oog ba...

Watch this Scientific Journal Video about A Laser-induced Mouse Model of Chronic Ocular Hypertension to Characterize Visual Defects at JoVE.com

A Laser-induced Mouse Model of Chronic Ocular Hypertension to Characterize Visual Defects

Chronische oculaire hypertensie wordt opgewekt met behulp van laserfotocoagulatie van de trabekelnetwerk in muis ogen. De intraoculaire druk (IOP) wordt verhoogd tot verscheidene maanden na laserbehandeling. De daling van de gezichtsscherpte en contrastgevoeligheid proefdieren worden gecontroleerd met behulp van de optomotor-test.

Een laser-induced Mouse Model van chronische oculaire hypertensie aan Visual Gebreken karakteriseren

Glaucoma, frequently associated with elevated intraocular pressure (IOP), is one of the leading causes of blindness. We sought to establish a mouse model ...

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A Reversible Silicon Oil-Induced Ocular Hypertension Model in Mice

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Elevated intraocular pressure (IOP) is a well-documented risk factor for glaucoma. Here we describe a novel, effective method for consistently inducing ...