Name: a laser-induced mouse model of chronic ocular hypertension to characterize visual defects
Uploaded: 2013-08-14T14:00:00
Description: Watch this Scientific Journal Video about A Laser-induced Mouse Model of Chronic Ocular Hypertension to Characterize Visual Defects at JoVE.com

Die Arbeit in diesem Papier enthaltenen Informationen wurden von der Dr. Douglas H. Johnson Award für Glaukomforschung vom American Health Assistance Foundation (XL), die William &amp; Mary Greve Besondere Scholar Award von der Forschung zur Verhütung Blindheit (XL), unterstützt die Illinois Gesellschaft zur Verhütung von Blindheit (HC) und NIH R01EY019034 (XL).

Verfahren C57BL/6J-Mäusen (Jackson Laboratory, Bar Harbor, ME) sind an der Northwestern University Animal Care Facility angehoben. Alle Tiere werden in Übereinstimmung mit den Protokollen von der Northwestern University Institutional Animal Care und Verwenden Ausschuss gestellt und den Leitlinien für die Verwendung von Tieren in Neuroscience Forschung vom NIH genehmigt werden. 1. Laserphotokoagulation Das Verfahren der Laserkoagulation ...

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Wir berichten über diesem anhaltenden Augenhypertension können durch Laser-Beleuchtung in Mäuseaugen induziert werden. Im Vergleich zur Injektion von Kochsalzlösung Modell 18 und der Vene cautery Modell 11 beide umfangreichen mikrochirurgischen Fähigkeiten erfordern, ist die Laserbestrahlung relativ einfach und leicht durchzuführen. Normalerweise können wir den Laser-Beleuchtung für 4-6 Mäuse in 2-3 Stunden durchzuführen. Die kritischen Schritte aufrechterhalten IOP Höhe erreichen sind d...

<table border="1">
 <tbody>
 <tr>
 <td></td>
 <td></td>
 <td></td>
 <td>Reagent</td>
 </tr>
 <tr>
 <td>moxifloxacin</td>
 <td>Alcon Labs, Inc.</td>
 <td>NDC 0065-4013-03</td>
 <td>0.5 %, Rx only</td>
 </tr>
 <tr>
 <td>Proparacaine Hydrochloride</td>
 <td>Bausch &amp; Lomb</td>
 <td>NDC 24208-730-06</td>
 <td>0.5 %, Rx only</td>
 </tr>
 <tr>
 <td>Ophthalmic Solution USP</td>
 <td>Bausch &amp; Lomb</td>
 <td>NDC 24208-730-06</td>
 <td>.5 %, Rx only</td>
 </tr>
 <tr>
 <td>ketamine</td>
 <td>Butler Schein Animal Health</td>
 <td>NDC 11695-0550-1</td>
 <td>100 mg / kg </td>
 </tr>
 <tr>
 <td>xylazine</td>
 <td>LLOYD Inc. of Iowa</td>
 <td>NADA 139-236</td>
 <td>10 mg / kg </td>
 </tr>
 <tr>
 <td>atropine sulfate solution</td>
 <td>Alcon Labs, Inc.</td>
 <td>NDC 61314-303-02</td>
 <td>1 %, Rx only</td>
 </tr>
 <tr>
 <td></td>
 <td></td>
 <td></td>
 <td>Equipment </td>
 </tr>
 <tr>
 <td>Slit Lamp, TOPCON </td>
 <td>Visual Systems Inc</td>
 <td>SL-3E</td>
 <td>powered by PS-30A</td>
 </tr>
 <tr>
 <td>OptoMotry 1.8.0 virtual</td>
 <td>CerebralMechanics Inc.</td>
 <td></td>
 <td></td>
 </tr>
 <tr>
 <td>opto-kinetic testing system</td>
 <td>CerebralMechanics Inc.</td>
 <td></td>
 <td></td>
 </tr>
 <tr>
 <td>Tonometer, TonoLab, for mice</td>
 <td>Colonial Medical Supply</td>
 <td></td>
 <td></td>
 </tr>
 <tr>
 <td>Heating pad</td>
 <td>Sunbeam Products Inc</td>
 <td>722-810</td>
 <td></td>
 </tr>
 <tr>
 <td>Argon laser </td>
 <td>Coherent Inc</td>
 <td>Ultima 2000SE</td>
 <td></td>
 </tr>
 <tr>
 <td>DECAPICONE Plastic cone holder </td>
 <td>Braintree Sci Inc.</td>
 <td>MDC-200</td>
 <td>for mouse</td>
 </tr>
 </tbody>
 </table>

a laser-induced mouse model of chronic ocular hypertension to characterize visual defects

Glaukom, häufig mit erhöhtem Augeninnendruck (IOP) verbunden sind, ist eine der führenden Ursachen für Erblindung. Wir versuchten, ein Maus-Modell von Augenhypertension die menschliche High-Tension Glaukom imitieren zu etablieren. Hier Laserbeleuchtung mit dem Hornhautrand, um die wässerige Abfluß photocoagulate, induzieren Engwinkel. Die Änderungen des IOP erfolgt über ein Rebound-Tonometer vor und nach der Laserbehandlung. Ein optomotor Verhaltens-Test wird verwendet, um die entsprechenden Änderungen in der visuellen Leistungsfähigkeit zu messen. Die repräsentatives Ergebnis von einer Maus, die nachhaltig IOP Erhebung nach der Laser-Beleuchtung entwickelt gezeigt. Eine verminderte Sehschärfe und Kontrastempfindlichkeit wird in diesem Augeninnendruck Maus beobachtet. Gemeinsam stellt unsere Studie einen wertvollen Modellsystem zur neuronalen Degeneration und die zugrunde liegenden molekularen Mechanismen in glaukomatöse Mäusen zu untersuchen.

Wie in den Verfahren beschrieben, ist die Laser-Beleuchtung an der Trabekelnetzwerk im Limbus Region, um die Kammerwasserabfluss photocoagulate Ziel, Induktion Winkel Verschluss (Abbildung 1). Die meisten gelaserten Augen zeigten keine erheblichen Sachbeschädigungen, Pigment Ablösung oder Infektion, im Einklang mit früheren Befunden 6. Wenn eine kleine Gruppe von Mäusen (weniger als 5% aller gelaserten Tiere) körperliche Anzeichen von schweren Schäden wie entleert Augäpfel, schwere Kat...

Watch this Scientific Journal Video about A Laser-induced Mouse Model of Chronic Ocular Hypertension to Characterize Visual Defects at JoVE.com

A Laser-induced Mouse Model of Chronic Ocular Hypertension to Characterize Visual Defects

Chronische Augenhypertension induziert wird mittels Laserkoagulation der Trabekelnetzwerk in Maus Augen. Der Augeninnendruck (IOP) wird für mehrere Monate nach der Laser-Behandlung erhöht. Die Abnahme der Sehschärfe und Kontrastempfindlichkeit von Versuchstieren werden anhand des optomotor Test.

Ein Laser-induzierten Maus-Modell der chronischen Ocular Hypertension zu Sehfehler Charakterisierung

Glaucoma, frequently associated with elevated intraocular pressure (IOP), is one of the leading causes of blindness. We sought to establish a mouse model ...

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The understanding of the pathophysiology of brain arteriovenous malformations and arteriovenous fistulas has improved thanks to animal models. A rat model ...

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Beurteilung der Gefäßfunktion bei Patienten mit chronischer Nierenerkrankung

Patients with chronic kidney disease (CKD) have significantly increased risk of cardiovascular disease (CVD) compared to the general population, and this ...

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The mouse laser-induced choroidal neovascularization (CNV) model has been a crucial mainstay model for neovascular age-related macular degeneration (AMD) ...

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Ein Magnet Microbead Occlusion Modell Ocular Hypertension Abhängige Glaucoma in Mäusen zu induzieren

The use of rodent models of glaucoma has been essential to understand the molecular mechanisms that underlie the pathophysiology of this multifactorial ...

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Eine einfache mechanische Verfahren Limbusstammzellinsuffizienzen in der Maus zum Erstellen

Limbal stem cell deficiency (LSCD) is a state of malfunction or loss of limbal epithelial stem cells, after which the corneal epithelium is replaced with ...

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Induktion und Phänotypisierung der akuten Rechtsherzinsuffizienz in einem Großtiermodell der chronischen thromboembolischen pulmonalen Hypertonie

The development of acute right heart failure (ARHF) in the context of chronic pulmonary hypertension (PH) is associated with poor short-term outcomes. The ...

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Messung der isometrischen Kontraktilität der Maus mesenterialen Arterie mit Draht Myographie

The wire myograph technique is used to assess the contractility of vascular smooth muscles in response to depolarization, GPCR agonists/inhibitors and ...

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Ein reversibles Siliziumöl-induziertes Okularhypertonie-Modell bei Mäusen

Elevated intraocular pressure (IOP) is a well-documented risk factor for glaucoma. Here we describe a novel, effective method for consistently inducing ...