Name: a laser-induced mouse model of chronic ocular hypertension to characterize visual defects
Uploaded: 2013-08-14T14:00:00
Description: Watch this Scientific Journal Video about A Laser-induced Mouse Model of Chronic Ocular Hypertension to Characterize Visual Defects at JoVE.com

Arbeidet i dette papiret har blitt støttet av Dr. Douglas H. Johnson Award for Glaukom Forskning fra American Health Assistance Foundation (XL), William &amp; Mary Greve Special Scholar Award fra Forskningsrådet for å hindre blindhet (XL), den Illinois Society for Prevention of Blindness (HC) og NIH stipend R01EY019034 (XL).

Prosedyrer C57BL/6J mus (Jackson Laboratory, Bar Harbor, ME) er oppvokst ved Northwestern University Animal Care Facility. Alle dyr blir brukt i samsvar med protokoller godkjent av Northwestern University Institutional Animal Care og bruk Committee og konform til retningslinjer for bruk av dyr i nevrovitenskap forskning fra NIH. En. Laser fotokoagulasjons Prosedyren for laser fotokoagulasjon er endret fra tidligere publiserte protokoller <...

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Vi rapporterer over at vedvarende okulær hypertensjon kan være forårsaket av laser belysning i mus øyne. Sammenlignet med saltvann injeksjon modell 18 og venen cautery modell 11 som begge krever omfattende mikrokirurgisk ferdigheter, er laser belysning forholdsvis enkel og lett å utføre. Vanligvis kan vi utføre laser belysning for 4-6 mus i 2-3 timer. De kritiske skritt for å oppnå vedvarende IOP heving er den fremre kammer utflating før laser og parametrene for laser belysning. Drenering...

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 <td></td>
 <td></td>
 <td></td>
 <td>Reagent</td>
 </tr>
 <tr>
 <td>moxifloxacin</td>
 <td>Alcon Labs, Inc.</td>
 <td>NDC 0065-4013-03</td>
 <td>0.5 %, Rx only</td>
 </tr>
 <tr>
 <td>Proparacaine Hydrochloride</td>
 <td>Bausch &amp; Lomb</td>
 <td>NDC 24208-730-06</td>
 <td>0.5 %, Rx only</td>
 </tr>
 <tr>
 <td>Ophthalmic Solution USP</td>
 <td>Bausch &amp; Lomb</td>
 <td>NDC 24208-730-06</td>
 <td>.5 %, Rx only</td>
 </tr>
 <tr>
 <td>ketamine</td>
 <td>Butler Schein Animal Health</td>
 <td>NDC 11695-0550-1</td>
 <td>100 mg / kg </td>
 </tr>
 <tr>
 <td>xylazine</td>
 <td>LLOYD Inc. of Iowa</td>
 <td>NADA 139-236</td>
 <td>10 mg / kg </td>
 </tr>
 <tr>
 <td>atropine sulfate solution</td>
 <td>Alcon Labs, Inc.</td>
 <td>NDC 61314-303-02</td>
 <td>1 %, Rx only</td>
 </tr>
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 <td></td>
 <td></td>
 <td></td>
 <td>Equipment </td>
 </tr>
 <tr>
 <td>Slit Lamp, TOPCON </td>
 <td>Visual Systems Inc</td>
 <td>SL-3E</td>
 <td>powered by PS-30A</td>
 </tr>
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 <td>OptoMotry 1.8.0 virtual</td>
 <td>CerebralMechanics Inc.</td>
 <td></td>
 <td></td>
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 <td>opto-kinetic testing system</td>
 <td>CerebralMechanics Inc.</td>
 <td></td>
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 </tr>
 <tr>
 <td>Tonometer, TonoLab, for mice</td>
 <td>Colonial Medical Supply</td>
 <td></td>
 <td></td>
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 <tr>
 <td>Heating pad</td>
 <td>Sunbeam Products Inc</td>
 <td>722-810</td>
 <td></td>
 </tr>
 <tr>
 <td>Argon laser </td>
 <td>Coherent Inc</td>
 <td>Ultima 2000SE</td>
 <td></td>
 </tr>
 <tr>
 <td>DECAPICONE Plastic cone holder </td>
 <td>Braintree Sci Inc.</td>
 <td>MDC-200</td>
 <td>for mouse</td>
 </tr>
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 </table>

a laser-induced mouse model of chronic ocular hypertension to characterize visual defects

Glaukom, ofte assosiert med forhøyet intraokulært trykk (IOP), er en av de viktigste årsakene til blindhet. Vi har forsøkt å etablere en musemodell av okulær hypertensjon å etterligne humant høyspent glaukom. Her laser belysning brukes på hornhinnen limbus å photocoagulate den vandige strøm, indusere vinkel nedleggelse. Endringene i intraokulært trykk blir overvåket ved hjelp av en etterpå tonometer før og etter laserbehandling. En optomotor atferdsmessige testen brukes til å måle tilsvarende endringer i visuell kapasitet. Representanten resultat fra en mus som utviklet vedvarende IOP heving etter laser belysning vises. En redusert synsskarphet og kontrast følsomhet er observert i dette okulær hypertensjon mus. Sammen introduserer vår studie en verdifull modellsystem for å undersøke neuronal degenerasjon og de underliggende molekylære mekanismer i glaucomatous mus.

Som beskrevet i de prosedyrer, er laser-lys rettet mot den trabekelverket i limbale regionen til photocoagulate den vandige utstrømningen, indusere vinkel lukning (fig. 1). Mest lasered øyne viste ingen betydelig fysisk skade, pigment avløsning eller infeksjon, i tråd med tidligere funn seks. Når en liten gruppe mus (mindre enn 5% av alle lasered dyr) viste fysiske tegn på alvorlig skade som punkterte øye baller, alvorlig katarakt, betydelig pigment avløsning, eller blødning, avlivet...

Watch this Scientific Journal Video about A Laser-induced Mouse Model of Chronic Ocular Hypertension to Characterize Visual Defects at JoVE.com

A Laser-induced Mouse Model of Chronic Ocular Hypertension to Characterize Visual Defects

Kronisk okulær hypertensjon er indusert ved hjelp av laser fotokoagulasjon av trabekelverket i mus øyne. Det intraokulære trykket (IOP) er forhøyet i flere måneder etter laserbehandling. Nedgangen av synsskarphet og kontrast følsomhet av forsøksdyr er overvåket ved hjelp av optomotor test.

En laser-indusert Mouse Modell av kronisk okulær hypertensjon å karakterisere Visuelle defekter

Glaucoma, frequently associated with elevated intraocular pressure (IOP), is one of the leading causes of blindness. We sought to establish a mouse model ...

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Elevated intraocular pressure (IOP) is a well-documented risk factor for glaucoma. Here we describe a novel, effective method for consistently inducing ...