Name: a laser-induced mouse model of chronic ocular hypertension to characterize visual defects
Uploaded: 2013-08-14T14:00:00
Description: Watch this Scientific Journal Video about A Laser-induced Mouse Model of Chronic Ocular Hypertension to Characterize Visual Defects at JoVE.com

O trabalho contido neste artigo tem sido apoiada pelo Prêmio Johnson Dr. Douglas H. de Pesquisa Glaucoma da American Assistance Foundation Saúde (XL), o William &amp; Mary Greve Scholar Award Especial da Investigação para Prevenir Cegueira (XL), o Illinois Sociedade para a Prevenção da Cegueira (HC) e NIH conceder R01EY019034 (XL).

Procedimentos Camundongos C57BL/6J (Jackson Laboratory, Bar Harbor, ME) são levantadas a Animal Care Facility da Universidade Northwestern. Todos os animais são utilizados em conformidade com os protocolos aprovados pela Northwestern University Animal Care Institucional e Comitê de Uso e conformados com as orientações sobre o uso de animais em pesquisa Neurociência do NIH. 1. Fotocoagulação a laser O procedimento de fotocoagulaçã...

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Descrevemos anteriormente que a hipertensão ocular sustentada pode ser induzida pela iluminação do laser em olhos de rato. Em comparação com o modelo de injecção de solução salina 18 e o modelo cautério veia 11 ambos os quais requerem extensivas técnicas de microcirurgia, a iluminação laser é relativamente simples e fácil de executar. Normalmente, podemos realizar a iluminação a laser para 4-6 ratos em 2-3 horas. Os passos fundamentais para alcançar elevação da PIO sustentado s?...

<table border="1">
 <tbody>
 <tr>
 <td></td>
 <td></td>
 <td></td>
 <td>Reagent</td>
 </tr>
 <tr>
 <td>moxifloxacin</td>
 <td>Alcon Labs, Inc.</td>
 <td>NDC 0065-4013-03</td>
 <td>0.5 %, Rx only</td>
 </tr>
 <tr>
 <td>Proparacaine Hydrochloride</td>
 <td>Bausch &amp; Lomb</td>
 <td>NDC 24208-730-06</td>
 <td>0.5 %, Rx only</td>
 </tr>
 <tr>
 <td>Ophthalmic Solution USP</td>
 <td>Bausch &amp; Lomb</td>
 <td>NDC 24208-730-06</td>
 <td>.5 %, Rx only</td>
 </tr>
 <tr>
 <td>ketamine</td>
 <td>Butler Schein Animal Health</td>
 <td>NDC 11695-0550-1</td>
 <td>100 mg / kg </td>
 </tr>
 <tr>
 <td>xylazine</td>
 <td>LLOYD Inc. of Iowa</td>
 <td>NADA 139-236</td>
 <td>10 mg / kg </td>
 </tr>
 <tr>
 <td>atropine sulfate solution</td>
 <td>Alcon Labs, Inc.</td>
 <td>NDC 61314-303-02</td>
 <td>1 %, Rx only</td>
 </tr>
 <tr>
 <td></td>
 <td></td>
 <td></td>
 <td>Equipment </td>
 </tr>
 <tr>
 <td>Slit Lamp, TOPCON </td>
 <td>Visual Systems Inc</td>
 <td>SL-3E</td>
 <td>powered by PS-30A</td>
 </tr>
 <tr>
 <td>OptoMotry 1.8.0 virtual</td>
 <td>CerebralMechanics Inc.</td>
 <td></td>
 <td></td>
 </tr>
 <tr>
 <td>opto-kinetic testing system</td>
 <td>CerebralMechanics Inc.</td>
 <td></td>
 <td></td>
 </tr>
 <tr>
 <td>Tonometer, TonoLab, for mice</td>
 <td>Colonial Medical Supply</td>
 <td></td>
 <td></td>
 </tr>
 <tr>
 <td>Heating pad</td>
 <td>Sunbeam Products Inc</td>
 <td>722-810</td>
 <td></td>
 </tr>
 <tr>
 <td>Argon laser </td>
 <td>Coherent Inc</td>
 <td>Ultima 2000SE</td>
 <td></td>
 </tr>
 <tr>
 <td>DECAPICONE Plastic cone holder </td>
 <td>Braintree Sci Inc.</td>
 <td>MDC-200</td>
 <td>for mouse</td>
 </tr>
 </tbody>
 </table>

a laser-induced mouse model of chronic ocular hypertension to characterize visual defects

Glaucoma, frequentemente associada com a pressão intra-ocular elevada (PIO), é uma das principais causas de cegueira. Buscou-se estabelecer um modelo do rato de hipertensão ocular para imitar alta tensão humana glaucoma. Aqui iluminação laser é aplicado ao limbo corneano para photocoagulate o escoamento do humor aquoso, induzindo fechamento angular. As mudanças de pressão intra-ocular são monitorizadas utilizando um tonómetro rebote antes e após o tratamento com laser. Um teste comportamental optomotor é usado para medir as mudanças correspondentes na capacidade visual. O resultado representante de um rato que desenvolveu elevação da PIO sustentada após a iluminação a laser é mostrado. Uma diminuição da acuidade visual e sensibilidade ao contraste é observado neste rato hipertenso ocular. Juntos, nosso estudo apresenta um modelo de sistema valioso para investigar a degeneração neuronal e dos mecanismos moleculares subjacentes em camundongos portadores de glaucoma.

Tal como descrito nos Procedimentos, iluminação laser é apontado para a malha trabecular na região límbica para photocoagulate o escoamento do humor aquoso, induzindo ângulo fechado (Figura 1). Olhos mais lasered exibiu nenhum dano físico significativo, descolamento pigmento ou infecção, de acordo com os achados anteriores 6. Quando um pequeno grupo de ratos (menos de 5% de todos os animais lasered) apresentaram sinais de danos físicos graves, como bolas deflacionados oculares grave...

Watch this Scientific Journal Video about A Laser-induced Mouse Model of Chronic Ocular Hypertension to Characterize Visual Defects at JoVE.com

A Laser-induced Mouse Model of Chronic Ocular Hypertension to Characterize Visual Defects

Hipertensão ocular crônica é induzida pelo uso de fotocoagulação a laser da malha trabecular em rato olhos. A pressão intra-ocular (IOP) é ​​elevada durante vários meses após o tratamento com laser. A diminuição da acuidade visual e sensibilidade ao contraste de animais experimentais são monitorados por meio do teste optomotor.

Um modelo de rato induzida por laser de crônica Ocular Hypertension para Caracterizar defeitos visuais

Glaucoma, frequently associated with elevated intraocular pressure (IOP), is one of the leading causes of blindness. We sought to establish a mouse model ...

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The development of acute right heart failure (ARHF) in the context of chronic pulmonary hypertension (PH) is associated with poor short-term outcomes. The ...

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The wire myograph technique is used to assess the contractility of vascular smooth muscles in response to depolarization, GPCR agonists/inhibitors and ...

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Elevated intraocular pressure (IOP) is a well-documented risk factor for glaucoma. Here we describe a novel, effective method for consistently inducing ...