Name: a laser-induced mouse model of chronic ocular hypertension to characterize visual defects
Uploaded: 2013-08-14T14:00:00
Description: Watch this Scientific Journal Video about A Laser-induced Mouse Model of Chronic Ocular Hypertension to Characterize Visual Defects at JoVE.com

Arbetet i denna uppsats har stöd av Dr Douglas H. Johnson Award för Glaukom Research från American Health Assistance Foundation (XL), William &amp; Mary Greve Special Scholar Award från Forskning kring att förebygga blindhet (XL), den Illinois Society för förebyggande av blindhet (HC) och NIH bidrag R01EY019034 (XL).

Förfaranden C57BL/6J-möss (Jackson Laboratory, Bar Harbor, ME) höjs vid Northwestern University Animal Care Facility. Alla djur används i enlighet med protokoll som godkänts av Northwestern University Institutional Animal Care och användning kommittén och överensstämmer med riktlinjerna för användning av djur i NeuroscienceForskning från NIH. Ett. Laserfotokoagulation Förfarandet i laserfotokoagulation är modifierad från tid...

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Vi rapporterar över den ihållande okulär hypertension kan induceras genom laserbelysning i mus ögon. Jämfört med den injektion av koksaltlösning modellen 18 och venen cautery modell 11 som båda kräver omfattande mikrokirurgiska färdigheter är laserbelysning relativt enkel och lätt att utföra. Vanligtvis kan vi utföra laserbelysning för 4-6 möss i 2-3 timmar. De kritiska stegen för att uppnå uthållig IOP höjd är den främre kammaren plattas innan laser och parametrarna för lase...

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 <td></td>
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 <td></td>
 <td>Reagent</td>
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 <td>moxifloxacin</td>
 <td>Alcon Labs, Inc.</td>
 <td>NDC 0065-4013-03</td>
 <td>0.5 %, Rx only</td>
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 <td>Proparacaine Hydrochloride</td>
 <td>Bausch &amp; Lomb</td>
 <td>NDC 24208-730-06</td>
 <td>0.5 %, Rx only</td>
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 <td>Ophthalmic Solution USP</td>
 <td>Bausch &amp; Lomb</td>
 <td>NDC 24208-730-06</td>
 <td>.5 %, Rx only</td>
 </tr>
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 <td>ketamine</td>
 <td>Butler Schein Animal Health</td>
 <td>NDC 11695-0550-1</td>
 <td>100 mg / kg </td>
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 <td>xylazine</td>
 <td>LLOYD Inc. of Iowa</td>
 <td>NADA 139-236</td>
 <td>10 mg / kg </td>
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 <td>atropine sulfate solution</td>
 <td>Alcon Labs, Inc.</td>
 <td>NDC 61314-303-02</td>
 <td>1 %, Rx only</td>
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 <td></td>
 <td>Equipment </td>
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 <td>Slit Lamp, TOPCON </td>
 <td>Visual Systems Inc</td>
 <td>SL-3E</td>
 <td>powered by PS-30A</td>
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 <td>OptoMotry 1.8.0 virtual</td>
 <td>CerebralMechanics Inc.</td>
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 <td>opto-kinetic testing system</td>
 <td>CerebralMechanics Inc.</td>
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 <td>Tonometer, TonoLab, for mice</td>
 <td>Colonial Medical Supply</td>
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 <td>Heating pad</td>
 <td>Sunbeam Products Inc</td>
 <td>722-810</td>
 <td></td>
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 <td>Argon laser </td>
 <td>Coherent Inc</td>
 <td>Ultima 2000SE</td>
 <td></td>
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 <td>DECAPICONE Plastic cone holder </td>
 <td>Braintree Sci Inc.</td>
 <td>MDC-200</td>
 <td>for mouse</td>
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a laser-induced mouse model of chronic ocular hypertension to characterize visual defects

Glaukom, ofta förenat med förhöjt intraokulärt tryck (lOP), är en av de främsta orsakerna till blindhet. Vi försökte etablera en musmodell av okulär hypertension för att efterlikna människans hög spänning glaukom. Här laserbelysning appliceras på hornhinnans limbus att photocoagulate det vattenhaltiga utflödet, inducera glaukom. Förändringarna i lOP övervakas med hjälp en rekyl tonometer före och efter laserbehandling. En optomotor beteendemässiga test används för att mäta motsvarande förändringar i visuell kapacitet. Den representativa resultat från en mus som utvecklade ihållande IOP höjd efter laserbelysning visas. En försämrad synskärpa och kontrast känslighet observeras i denna okulära hypertensiva mus. Tillsammans, introducerar vår studie en värdefull modell för att undersöka neuronal degeneration och de bakomliggande molekylära mekanismerna i glaukomatösa möss.

Som beskrivits i förfarandena, är laserbelysning syftar trabekelverket i limbus regionen att photocoagulate den vattenbaserade utflödet, förmå glaukom (Figur 1). Mest lasered ögon uppvisade ingen signifikant fysiska skador, pigment lossnar eller infektion, vilket överensstämmer med tidigare fynd 6. När en liten grupp av möss (mindre än 5% av alla lasered djur) uppvisade fysiska tecken på allvarliga skador såsom tömda öga bollar, svår katarakt, betydande pigment avskildhet, ell...

Watch this Scientific Journal Video about A Laser-induced Mouse Model of Chronic Ocular Hypertension to Characterize Visual Defects at JoVE.com

A Laser-induced Mouse Model of Chronic Ocular Hypertension to Characterize Visual Defects

Kronisk okulär hypertension induceras med laserfotokoagulation av trabekelverket i mus ögon. Den intraokulära trycket (lOP) är förhöjd i flera månader efter laserbehandling. Minskningen av synskärpa och kontrastkänslighet av försöksdjur övervakas med användning av optomotor testet.

En Laser-inducerad musmodell av kronisk okulär hypertension för att karaktärisera synfel

Glaucoma, frequently associated with elevated intraocular pressure (IOP), is one of the leading causes of blindness. We sought to establish a mouse model ...

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