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DOI :
10.3791/50448-v
June 25th, 2013
Chapters
0:05
Title
2:10
Construction of MBP-Mgm101 Fusion Protein
3:16
Expression, Induction, Cell Lysis and DNase I Treatment
5:28
Purification on Amylose Column
7:07
PreScission Protease Cleavage and Cation Exchange Chromatography
8:42
Size Exclusion Chromatography
10:27
Analysis of Purified Mgm101
11:23
Conclusion
酵母ミトコンドリア核様体タンパク質、Mgm101は、大きなオリゴマーの環を形成するRAD52型組換えタンパク質である。プロトコルは、陽イオン交換およびサイズ排除クロマトグラフィーと連結マルトース結合タンパク質(MBP)タギング方式を使用して可溶性組換えMgm101を調製するために記載されている。
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