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DOI :
10.3791/50455-v
August 13th, 2013
Chapters
0:05
Title
2:03
Cloning of a Gene of Interest into an Entry Vector
3:16
Generation of the Primary Virus Lysate
5:47
Virus Amplification
7:34
T Cell Infection
9:01
T Cell Activation and Polarization
10:01
T Cell Fixation Staining for Flow Cytometry
11:51
Infection Does Not Interfere with T Cell Activation
14:42
Conclusion
ナイーブCD4コクサッキーアデノウイルス受容体のトランスジェニック発現とT細胞へのアデノウイルス遺伝子導入は、制御性T細胞分化の分子解析を可能に in vitroで。
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