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DOI :
10.3791/50456-v
July 18th, 2013
Chapters
0:05
Title
1:36
Obtain Fluorescent Microscopy Movies of Single Yeast Cells Growing in a Microfluidic Chamber
6:33
Format and Segment Data for Tracking Using the FormatData GUI in MATLAB
9:00
Track Cells and Lineages through Time with ProcessTimeSeries GUI, and Curate ID and Lineage Assignments
12:33
Exporting Data for Analysis
13:35
Results: Analysis of Single-cell Time Series
16:22
Conclusion
我々は、単一セルの時系列データを抽出するために蛍光成長酵母細胞の顕微鏡ムービー、GUIベースのソフトウェアパッケージを取得する単純なプロトコルを提示する。分析では、目視検査と追跡対象データの手動キュレーションと統合され自動化された系統と分裂時間の割り当てが含まれています。
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