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DOI :
10.3791/50460-v
•
11:12 min
August 6th, 2013
Chapters
0:05
Title
2:16
Decellularization of the BioVaSc
3:52
Isolation of Primary Human Dermal Microvascular Endothelial Cells (mvECs) and Fibroblasts
5:50
Tumor Test System A. Static Culture
7:36
Tumor Test System B. Dynamic Culture
8:45
Results: Immunohistological Characterization of 3D Tumor Models
10:40
Conclusion
試験系として、ヒトの3D腫瘍組織を作成するための方法が記載されている。これらの技術は、脱細胞化生体血管柄足場(BioVaSc)、初代ヒト細胞およびフローバイオリアクターにおける動的条件下で、ならびに、静的条件下で培養することができる腫瘍細胞株に基づいている。
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