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26.3K Views
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09:39 min
June 26th, 2013
DOI :
10.3791/50483-v
Chapters
0:05
Title
1:29
Preparation of the Electrophysiological Rig and the Brain Slice Recording Chamber
2:27
Brain Removal and Hippocampal Dissection
4:51
Hippocampal Slices Preparation
5:56
Incubation of Slices in the Chamber
7:02
Results: Induction of LTP in Hippocampal Slices
9:04
Conclusion
Summary
本文提出了一种完整的方法来准备和保存在体外急性成年小鼠海马脑片。该协议允许非常稳定持久的长时程增强(LTP)的记录超过8小时,95%的成功率。
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