Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Please note that all translations are automatically generated. Click here for the English version.
DOI :
10.3791/50535-v
September 28th, 2013
Chapters
0:05
Title
1:35
Plasma Membrane Purification
3:56
Detergent Resistant Membrane Preparation
5:33
Extraction of Proteins from the Detergent Resistant Fraction and In-solution Trypsin Digestion
7:04
Use of C18-StageTips for Peptide Desalting and Concentration
8:48
Results: Comparative Proteomic Analysis
11:00
Conclusion
在这里,我们描述了植物细胞质膜的基础上,未标记的混合物分馏成耐洗涤剂和清洁剂的可溶性和膜在体内完全15 N标记的拟南芥细胞培养一个强大的方法。该过程被施加于比较蛋白质组学研究,了解信令进程。
Tags
-- Views
Related Videos
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Overview
Publishing Process
Editorial Board
Scope and Policies
Peer Review
FAQ
Submit
Librarians
Testimonials
Subscriptions
Access
Resources
Library Advisory Board
ABOUT JoVE
Leadership
Copyright © 2024 MyJoVE Corporation. All rights reserved