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DOI :
10.3791/50598-v
•
9:52 min
July 12th, 2013
Chapters
0:05
Title
1:58
Preparation of Targeting DNA Plasmid pGOI
2:48
Transfecting T. gondii Parasites
3:43
Selecting Transfected T. gondii Parasites
7:07
Deletion of HXGPRT Marker and C-terminal Tagging
7:48
Results: Deletion of rop18 and Removal of the HXGPRT Selectable Marker from gra2
8:54
Conclusion
כאן אנו מדווחים שיטה לשימוש בסוג I וסוג II Δku80 זנים של Toxoplasma gondii כדי לייצר ביעילות מחיקות גן ממוקדת והחלפות גן לאנליזה גנומית תפקודית.
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