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DOI :
10.3791/50598-v
•
9:52 min
July 12th, 2013
Chapters
0:05
Title
1:58
Preparation of Targeting DNA Plasmid pGOI
2:48
Transfecting T. gondii Parasites
3:43
Selecting Transfected T. gondii Parasites
7:07
Deletion of HXGPRT Marker and C-terminal Tagging
7:48
Results: Deletion of rop18 and Removal of the HXGPRT Selectable Marker from gra2
8:54
Conclusion
여기에서 우리는 유형 I 및 유형 II를 사용하는 방법을보고 Δku80 변종 톡소 포자충 효율적으로 기능 게놈 분석 대상으로 유전자 삭제 및 유전자 교체를 생성합니다.
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