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DOI :
10.3791/50606-v
June 19th, 2013
Chapters
0:05
Title
1:59
Generating the GPRS Packaging Cell Line: A Tet-dependent SVGmu Cell Line
5:33
Constructing DC-LV Producer Cells by Concatemer Array Transfection
10:54
Producing and Concentrating Lentiviral Vectors
12:16
Results: Isolation of High-titer Lentiviral Vectors that Specifically Target Dendritic Cells
15:15
Conclusion
9:14
Evaluating Viral Production of Each Cell Clone
ここでは、レトロウイルス形質導入およびテトラサイクリンの不存在下でレンチウイルスベクター(LV)の成分を発現できる細胞株を作成するためのコンカテマートランスフェクションを使用する。このLVは、GFPをコードし、樹状細胞上の受容体に特異的な糖タンパク質、SVGmuと偽されています。
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