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DOI :
10.3791/50607-v
•
10:59 min
October 18th, 2014
Chapters
0:05
Title
1:24
Preparation of the CometChip
2:20
Loading Cells into the CometChip
3:57
Dosing and Repair
4:55
Alkaline Comet Assay
6:23
Neutral Comet Assay
7:50
Fluorescent Imaging
8:21
Results: Quantification of DNA Damage in Human Lymphoblastoid Cells after Exposure to Oxidative Damage
10:15
Conclusion
我们在这里描述了一个平台,使彗星试验检测DNA损伤了前所未有的吞吐量。该器件的图案哺乳动物细胞成芯片,使96个样品进行并行处理。这种方法有利于基础水平的DNA损伤,暴露诱导的DNA损伤和DNA修复的动力学分析。
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