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DOI :
10.3791/50607-v
•
10:59 min
October 18th, 2014
Chapters
0:05
Title
1:24
Preparation of the CometChip
2:20
Loading Cells into the CometChip
3:57
Dosing and Repair
4:55
Alkaline Comet Assay
6:23
Neutral Comet Assay
7:50
Fluorescent Imaging
8:21
Results: Quantification of DNA Damage in Human Lymphoblastoid Cells after Exposure to Oxidative Damage
10:15
Conclusion
ここでは前例のないスループットDNA損傷のコメットアッセイの検出を可能にするプラットフォームについて説明します。デバイスパターンマイクロアレイへの哺乳動物細胞および96サンプルの並列処理を可能にします。アプローチは、基本レベルのDNA損傷、曝露によって誘導されるDNA損傷とDNA修復の動態の解析を容易にする。
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