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9.5K Views
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08:21 min
September 4th, 2013
DOI :
10.3791/50612-v
Chapters
0:05
Title
1:20
Development of LabView Program
2:31
Preparation of Equipment
3:08
Preparation of the Gas Jet Capillary Nozzle Height
3:46
Cell Permeabilization Protocol
6:21
Results: Helium Gas Jet Creates Micropores in the Cell Membrane
7:57
Conclusion
Summary
这个协议描述了用于贴壁细胞用惰性气体喷射的暂时通透性的方法。这种技术有利于遗传物质和生物分子转移到贴壁哺乳动物细胞中通过机械力的利用来破坏细胞膜。
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