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07:44 min
October 1st, 2013
DOI :
10.3791/50628-v
Chapters
0:05
Title
0:57
Sample Preparation
2:03
Cell Disruption and Lipid Extraction
3:44
Transesterification to fatty Acid Methyl Esters
4:51
Quantification of Fatty Acid Methyl Esters Using Gas Chromatography
5:25
Results: Fatty Acid Content and Composition of Microalgae as Determined by Gas Chromatography
7:19
Conclusion
Summary
一种用于脂肪酸含量和组成在微藻基于机械破碎细胞,溶剂为基础的脂质提取,酯交换,以及量化和鉴定用气相色谱法的脂肪酸的测定方法进行说明。一个tripentadecanoin内标是用来弥补提取和不完整的酯交换过程中可能发生的损失。
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