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DOI :
10.3791/50633-v
July 22nd, 2013
Chapters
0:05
Title
2:48
Growth of Cells and Preparation for Imaging
3:29
Mito-roGFP1 Imaging
4:33
MitGO-ATeam2 Imaging
8:40
Measuring Mitochondrial Redox State and ATP with Mito-roGFP1 and MitGO-ATeam2
11:22
Conclusion
6:09
ImageJ Analysis
우리는 살아있는 효모 세포에서 세포 내 해상도에서 미토콘드리아의 산화 환원 상태와 ATP 수준을 모니터링하기 위해, GFP를 기반으로하는 두 개의 비례, 유전자 인코딩 바이오 센서의 사용을 설명합니다.
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