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DOI :
10.3791/50635-v
•
7:49 min
September 9th, 2013
Chapters
0:05
Title
1:09
Recrystallization of Matrices in order to Improve Their Purity (Optional)
2:06
Cleaning of MALDI Stainless Steel Target
2:49
α-CHCA Thin Layer Solution: Preparation and Deposition on the MALDI Target
3:18
Preparation of Matrix Solutions: α-CHCA, DHB, and CHCA_DHB Mixture
4:04
Sample Deposition on the MALDI Target and MALDI Spectra Acquisition
5:17
Results: MALDI-TOF Analysis of Proteins Larger than 100 kDa
7:08
Conclusion
精确质量测定代表蛋白质的调查过程中的重要一步。基质辅助激光解吸/电离(MALDI)质谱(MS)可用于这种确定和其主要优点是耐受的盐,去垢剂和污染物。在这里,我们示出了用于蛋白质大于100 kDa的通过MALDI-MS较大的分析可访问的方法。
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