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DOI :
10.3791/50646-v
October 8th, 2013
Chapters
0:05
Title
2:05
Obtaining Biopsy of Human NECs
3:37
Seeding NECs on Plastic
4:41
Expanding the Cells in Flasks
6:00
Seeding Cells on Tissue Culture Inserts
7:53
Establishing Air Liquid Interface (ALI)
8:35
ALI Culture of NECs Produces a Differentiated Epithelium
9:43
Conclusion
ヒトボランティアの表面掻き取り、生検により得られた鼻上皮細胞は、組織培養インサートに展開され、転送される。コンフルエントに達したら、細胞は、繊毛および非繊毛細胞の培養物を得、空気液体界面で成長させる。差別化された鼻の上皮細胞培養物は、呼吸器粘膜を研究するための実行可能な実験モデルを提供する。
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