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DOI :
10.3791/50647-v
August 9th, 2013
Chapters
0:05
Title
1:20
Neonatal Microglia Dissection: Day 0
4:24
Cell Culture: Day 0
5:31
Cell Culture: Day 10
6:40
Adult Microglia: Tissue Colection
9:07
Results: Representative Images of Neonatal and Adult Microglia
10:50
Conclusion
7:56
Digestion of Spinal Cord Tissue
我们概述方法从新生儿大脑皮层和脊髓成人的可行的小胶质细胞的高效和快速隔离/文化。解剖和电镀皮质小胶质细胞可以在90分钟内完成,与随后发生〜10天的初始剥离后的小胶质细胞的收获。
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