Transsynaptisk Tracing från perifera mål med pseudorabiesvirus Följt av koleratoxin och Biotinyliserad Dextran Amines dubbel märkning

Transsynaptic tracing has become a powerful tool for analyzing central efferents regulating peripheral targets through multi-synaptic circuits. Here we present a protocol that exploits the transsynaptic pseudorabies virus to identify and localize a functional brain circuit, followed by classical tract tracing techniques to validate specific connections in the circuit between identified groups of neurons.